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dc.contributor.authorXie, Wen
dc.contributor.authorChen, Chunhai
dc.contributor.authorYang, Zezhong
dc.contributor.authorGuo, Litao
dc.contributor.authorYang, Xin
dc.contributor.authorWang, Dan
dc.contributor.authorChen, Ming
dc.contributor.authorHuang, Jinqun
dc.contributor.authorWen, Yanan
dc.contributor.authorZeng, Yang
dc.contributor.authorLiu, Yating
dc.contributor.authorXia, Jixing
dc.contributor.authorTian, Lixia
dc.contributor.authorCui, Hongying
dc.contributor.authorWu, Qingjun
dc.contributor.authorWang, Shaoli
dc.contributor.authorXu, Baoyun
dc.contributor.authorLi, Xianchun
dc.contributor.authorTan, Xinqiu
dc.contributor.authorGhanim, Murad
dc.contributor.authorQiu, Baoli
dc.contributor.authorPan, Huipeng
dc.contributor.authorChu, Dong
dc.contributor.authorDelatte, Helene
dc.contributor.authorMaruthi, M. N.
dc.contributor.authorGe, Feng
dc.contributor.authorZhou, Xueping
dc.contributor.authorWang, Xiaowei
dc.contributor.authorWan, Fanghao
dc.contributor.authorDu, Yuzhou
dc.contributor.authorLuo, Chen
dc.contributor.authorYan, Fengming
dc.contributor.authorPreisser, Evan L.
dc.contributor.authorJiao, Xiaoguo
dc.contributor.authorCoates, Brad S.
dc.contributor.authorZhao, Jinyang
dc.contributor.authorGao, Qiang
dc.contributor.authorXia, Jinquan
dc.contributor.authorYin, Ye
dc.contributor.authorLiu, Yong
dc.contributor.authorBrown, Judith K.
dc.contributor.authorZhou, Xuguo “Joe”
dc.contributor.authorZhang, Youjun
dc.date.accessioned2017-07-17T16:38:58Z
dc.date.available2017-07-17T16:38:58Z
dc.date.issued2017-05
dc.identifier.citationGenome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q 2017, 6 (5):1 GigaScienceen
dc.identifier.issn2047-217X
dc.identifier.doi10.1093/gigascience/gix018
dc.identifier.urihttp://hdl.handle.net/10150/624730
dc.description.abstractThe sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.
dc.description.sponsorshipNational Natural Science Foundation of China [31420103919, 31672032]; Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) the China Agriculture Research System [CARS-26-10]; Beijing Training Project for the Leading Talents in S T [LJRC201412]; Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables; Beijing Nova Program [Z171100001117039]en
dc.language.isoenen
dc.publisherOXFORD UNIV PRESSen
dc.relation.urlhttps://academic.oup.com/gigascience/article-lookup/doi/10.1093/gigascience/gix018en
dc.rights© The Author 2017. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.en
dc.subjectWhitefly Bemisia tabacien
dc.subjectGenomicsen
dc.subjectAssemblyen
dc.subjectAnnotationen
dc.titleGenome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Qen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Sch Plant Scien
dc.identifier.journalGigaScienceen
dc.description.noteOpen Access Journal.en
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
refterms.dateFOA2018-08-16T10:59:10Z
html.description.abstractThe sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.


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