Measuring Environmental Chitin and Allergen Levels to Predict Pediatric Asthma Risk
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractOur primary objective in this project was to analyze dust samples obtained from the Inner-City Anti-IgE Therapy for Asthma study, a 60-week pediatric asthma study which evaluated changes in asthma symptoms in inner-city children during a treatment course with an experimental Anti-IgE medication. During the study, children submitted household dust samples for analysis. When the study was completed, we obtained these dust samples and developed a novel assay to quantify their levels of chitin, a common household allergen. Using statistical software, we compared clinical data obtained during the ICATA study to our analyses of the patient-matched dust samples. We found that dust sample chitin concentration significantly correlates with severity of self-reported sleep-interrupting and activity-limiting asthma symptoms with p-values of 0.001 and 0.018, respectively. We also found that dust mite allergen Der p 1 correlates to continuous and bimodal chitin with p-values 0.035 and 0.0474, respectively, and cockroach allergen Bla g 1 correlates to continuous and bimodal chitin with p-values 0.0016 and 0.0335, respectively. From these data, we conclude that our chitin assay may be a promising tool with which to evaluate the risk of asthma development in indoor environments.
Degree ProgramHonors College