Molecular evolutionary analysis of a gender-limited MID ortholog from the homothallic species Volvox africanus with male and monoecious spheroids
Ferris, Patrick J.
AffiliationUniv Arizona, Dept Ecol & Evolutionary Biol
MetadataShow full item record
PublisherPUBLIC LIBRARY SCIENCE
CitationMolecular evolutionary analysis of a gender-limited MID ortholog from the homothallic species Volvox africanus with male and monoecious spheroids 2017, 12 (6):e0180313 PLOS ONE
Rights© 2017 Yamamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
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AbstractVolvox is a very interesting oogamous organism that exhibits various types of sexuality and/or sexual spheroids depending upon species or strains. However, molecular bases of such sexual reproduction characteristics have not been studied in this genus. In the model species V. carteri, an ortholog of the minus mating type-determining or minus dominance gene (MID) of isogamous Chlamydomonas reinhardtii is male-specific and determines the sperm formation. Male and female genders are genetically determined (heterothallism) in V. carteri, whereas in several other species of Volvox both male and female gametes (sperm and eggs) are formed within the same clonal culture (homothallism). To resolve the molecular basis of the evolution of Volvox species with monoecious spheroids, we here describe a MID ortholog in the homothallic species V. africanus that produces both monoecious and male spheroids within a single clonal culture. Comparison of synonymous and nonsynonymous nucleotide substitutions in MID genes between V. africanus and heterothallic volvocacean species suggests that the MID gene of V. africanus evolved under the same degree of functional constraint as those of the heterothallic species. Based on semi quantitative reverse transcription polymerase chain reaction analyses using the asexual, male and monoecious spheroids isolated from a sexually induced V. africanus culture, the MID mRNA level was significantly upregulated in the male spheroids, but suppressed in the monoecious spheroids. These results suggest that the monoecious spheroid-specific down regulation of gene expression of the MID homolog correlates with the formation of both eggs and sperm in the same spheroid in V. africanus.
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VersionFinal published version
SponsorsMinistry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI [15K14590, 16H02518]; National Aeronautics and Space Administration [NNX13AH41G]; National Science Foundation [MCB-1412395]
- Delineating a New Heterothallic Species of Volvox (Volvocaceae, Chlorophyceae) Using New Strains of "Volvox africanus".
- Authors: Nozaki H, Matsuzaki R, Yamamoto K, Kawachi M, Takahashi F
- Issue date: 2015
- Morphology, taxonomy and mating-type loci in natural populations of Volvox carteri in Taiwan.
- Authors: Nozaki H, Ueki N, Takusagawa M, Yamashita S, Misumi O, Matsuzaki R, Kawachi M, Chiang YR, Wu JT
- Issue date: 2018 Apr 3
- Evolution of sexes from an ancestral mating-type specification pathway.
- Authors: Geng S, De Hoff P, Umen JG
- Issue date: 2014 Jul
- Evolutionary divergence of the sex-determining gene <i>MID</i> uncoupled from the transition to anisogamy in volvocine algae.
- Authors: Geng S, Miyagi A, Umen JG
- Issue date: 2018 Apr 9
- Evolution of an expanded sex-determining locus in Volvox.
- Authors: Ferris P, Olson BJ, De Hoff PL, Douglass S, Casero D, Prochnik S, Geng S, Rai R, Grimwood J, Schmutz J, Nishii I, Hamaji T, Nozaki H, Pellegrini M, Umen JG
- Issue date: 2010 Apr 16