High resolution, high speed, long working distance, large field of view confocal fluorescence microscope
Chawla, Monica K.
Baggett, Brend K.
Barnes, Carol A.
AffiliationUniv Arizona, Coll Opt Sci
Univ Arizona, Evelyn F McKnight Brain Inst
Univ Arizona, ARL Div Neural Syst
Univ Arizona, Dept Biomed Engn
Univ Arizona, Dept Psychol
Univ Arizona, Dept Neurol
Univ Arizona, Dept Neurosci
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationHigh resolution, high speed, long working distance, large field of view confocal fluorescence microscope 2017, 7 (1) Scientific Reports
Rights© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractConfocal fluorescence microscopy is often used in brain imaging experiments, however conventional confocal microscopes are limited in their field of view, working distance, and speed for high resolution imaging. We report here the development of a novel high resolution, high speed, long working distance, and large field of view confocal fluorescence microscope ((HL2)-L-2-CFM) with the capability of multiregion and multifocal imaging. To demonstrate the concept, a 0.5 numerical aperture (NA) confocal fluorescence microscope is prototyped with a 3 mm x 3 mm field of view and 12 mm working distance, an array of 9 beams is scanned over the field of view in 9 different regions to speed up the acquisition time by a factor of 9. We test this custom designed confocal fluorescence microscope for future use with brain clarification methods to image large volumes of the brain at subcellular resolution. This multiregion and multi-spot imaging method can be used in other imaging modalities, such as multiphoton microscopes, and the field of view can be extended well beyond 12 mm x 12 mm.
VersionFinal published version
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