Show simple item record

dc.contributor.authorPacheco, Shaun
dc.contributor.authorWang, Chengliang
dc.contributor.authorChawla, Monica K.
dc.contributor.authorNguyen, Minhkhoi
dc.contributor.authorBaggett, Brend K.
dc.contributor.authorUtzinger, Urs
dc.contributor.authorBarnes, Carol A.
dc.contributor.authorLiang, Rongguang
dc.date.accessioned2017-11-03T21:43:24Z
dc.date.available2017-11-03T21:43:24Z
dc.date.issued2017-10-17
dc.identifier.citationHigh resolution, high speed, long working distance, large field of view confocal fluorescence microscope 2017, 7 (1) Scientific Reportsen
dc.identifier.issn2045-2322
dc.identifier.pmid29042677
dc.identifier.doi10.1038/s41598-017-13778-2
dc.identifier.urihttp://hdl.handle.net/10150/625967
dc.description.abstractConfocal fluorescence microscopy is often used in brain imaging experiments, however conventional confocal microscopes are limited in their field of view, working distance, and speed for high resolution imaging. We report here the development of a novel high resolution, high speed, long working distance, and large field of view confocal fluorescence microscope ((HL2)-L-2-CFM) with the capability of multiregion and multifocal imaging. To demonstrate the concept, a 0.5 numerical aperture (NA) confocal fluorescence microscope is prototyped with a 3 mm x 3 mm field of view and 12 mm working distance, an array of 9 beams is scanned over the field of view in 9 different regions to speed up the acquisition time by a factor of 9. We test this custom designed confocal fluorescence microscope for future use with brain clarification methods to image large volumes of the brain at subcellular resolution. This multiregion and multi-spot imaging method can be used in other imaging modalities, such as multiphoton microscopes, and the field of view can be extended well beyond 12 mm x 12 mm.
dc.language.isoenen
dc.publisherNATURE PUBLISHING GROUPen
dc.relation.urlhttp://www.nature.com/articles/s41598-017-13778-2en
dc.rights© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleHigh resolution, high speed, long working distance, large field of view confocal fluorescence microscopeen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Coll Opt Scien
dc.contributor.departmentUniv Arizona, Evelyn F McKnight Brain Insten
dc.contributor.departmentUniv Arizona, ARL Div Neural Systen
dc.contributor.departmentUniv Arizona, Dept Biomed Engnen
dc.contributor.departmentUniv Arizona, Dept Psycholen
dc.contributor.departmentUniv Arizona, Dept Neurolen
dc.contributor.departmentUniv Arizona, Dept Neuroscien
dc.identifier.journalScientific Reportsen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
refterms.dateFOA2018-08-19T17:36:02Z
html.description.abstractConfocal fluorescence microscopy is often used in brain imaging experiments, however conventional confocal microscopes are limited in their field of view, working distance, and speed for high resolution imaging. We report here the development of a novel high resolution, high speed, long working distance, and large field of view confocal fluorescence microscope ((HL2)-L-2-CFM) with the capability of multiregion and multifocal imaging. To demonstrate the concept, a 0.5 numerical aperture (NA) confocal fluorescence microscope is prototyped with a 3 mm x 3 mm field of view and 12 mm working distance, an array of 9 beams is scanned over the field of view in 9 different regions to speed up the acquisition time by a factor of 9. We test this custom designed confocal fluorescence microscope for future use with brain clarification methods to image large volumes of the brain at subcellular resolution. This multiregion and multi-spot imaging method can be used in other imaging modalities, such as multiphoton microscopes, and the field of view can be extended well beyond 12 mm x 12 mm.


Files in this item

Thumbnail
Name:
s41598-017-13778-2.pdf
Size:
2.453Mb
Format:
PDF
Description:
FInal Published Version

This item appears in the following Collection(s)

Show simple item record

© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.