Quantitative microglia analyses reveal diverse morphologic responses in the rat cortex after diffuse brain injury
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Univ Arizona, Coll Med PhoenixUniv Arizona, Coll Nursing
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2017-10-16
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NATURE PUBLISHING GROUPCitation
Quantitative microglia analyses reveal diverse morphologic responses in the rat cortex after diffuse brain injury 2017, 7 (1) Scientific ReportsJournal
Scientific ReportsRights
© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Determining regions of altered brain physiology after diffuse brain injury is challenging. Microglia, brain immune cells with ramified and dynamically moving processes, constantly surveil the parenchyma for dysfunction which, when present, results in a changed morphology. Our purpose was to define the spatiotemporal changes in microglia morphology over 28 days following rat midline fluid percussion injury (mFPI) as a first step in exploiting microglia morphology to reflect altered brain physiology. Microglia morphology was quantified from histological sections using Image J skeleton and fractal analysis procedures at three time points and in three regions post-mFPI: impact site, primary somatosensory cortex barrel field (S1BF), and a remote region. Microglia ramification (process length/cell and endpoints/cell) decreased in the impact and S1BF but not the remote region (p < 0.05). Microglia complexity was decreased in the S1BF (p = 0.003) and increased in the remote region (p < 0.02). Rod-shaped microglia were present in the S1BF and had a 1.8:1.0 length: width ratio. An in-depth quantitative morphologic analysis revealed diverse and widespread changes to microglia morphology in the cortex post-mFPI. Due to their close link to neuronal function, changes in microglia morphology, summarized in this study, likely reflect altered physiology with diverse and widespread impact on neuronal and circuit function.Note
UA Open Access Publishing Fund.ISSN
2045-2322PubMed ID
29038483Version
Final published versionSponsors
National Institute of Neurological Disorders and Stroke of the National Institutes of Health [R21 NS096515, R03 NS090013]; Science Foundation Arizona Bisgrove Scholarship; PCH Mission Support FundsAdditional Links
http://www.nature.com/articles/s41598-017-13581-zae974a485f413a2113503eed53cd6c53
10.1038/s41598-017-13581-z
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Except where otherwise noted, this item's license is described as © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.
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