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Post-transcriptional Inhibition of Hsc70-4/HSPA8 Expression Leads to Synaptic Vesicle Cycling Defects in Multiple Models of ALS
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Author
Coyne, Alyssa N.Lorenzini, Ileana
Chou, Ching-Chieh
Torvund, Meaghan
Rogers, Robert S.
Starr, Alexander
Zaepfel, Benjamin L.
Levy, Jennifer
Johannesmeyer, Jeffrey
Schwartz, Jacob C.
Nishimune, Hiroshi
Zinsmaier, Konrad
Rossoll, Wilfried
Sattler, Rita
Zarnescu, Daniela C.
Affiliation
Univ Arizona, Dept Mol & Cellular BiolUniv Arizona, Dept Neurosci
Univ Arizona, Dept Neurol
Univ Arizona, Dept Chem & Biochem
Issue Date
2017-10
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CELL PRESSCitation
Post-transcriptional Inhibition of Hsc70-4/HSPA8 Expression Leads to Synaptic Vesicle Cycling Defects in Multiple Models of ALS 2017, 21 (1):110 Cell ReportsJournal
Cell ReportsRights
© 2017 The Author(s). This is an open access article under the CC BY-NC-ND license.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Amyotrophic lateral sclerosis (ALS) is a synaptopathy accompanied by the presence of cytoplasmic aggregates containing TDP-43, an RNA-binding protein linked to similar to 97% of ALS cases. Using a Drosophila model of ALS, we show that TDP-43 overexpression (OE) in motor neurons results in decreased expression of the Hsc70-4 chaperone at the neuromuscular junction (NMJ). Mechanistically, mutant TDP-43 sequesters hsc70-4 mRNA and impairs its translation. Expression of the Hsc70-4 ortholog, HSPA8, is also reduced in primary motor neurons and NMJs of mice expressing mutant TDP-43. Electrophysiology, imaging, and genetic interaction experiments reveal TDP-43-dependent defects in synaptic vesicle endocytosis. These deficits can be partially restored by OE of Hsc70-4, cysteine-string protein (Csp), or dynamin. This suggests that TDP-43 toxicity results in part from impaired activity of the synaptic CSP/Hsc70 chaperone complex impacting dynamin function. Finally, Hsc70-4/HSPA8 expression is also post-transcriptionally reduced in fly and human induced pluripotent stem cell (iPSC) C9orf72 models, suggesting a common disease pathomechanism.ISSN
22111247PubMed ID
28978466Version
Final published versionSponsors
NIH K-INBRE postdoctoral award [P20 GM103418]; NIH [R00 NS082376, R01NS078214, R01AG051470, R21 NS094809, RO1 NS091299]; Emory Medicine Catalyst Award; Muscular Dystrophy Association [MDA348086]; ALS Association ALSA [16-IIP-278]; Barrow Neurological Foundation; Himelic Family Foundation; Neuroscience Graduate Interdisciplinary Program at UA; ARCSAdditional Links
http://linkinghub.elsevier.com/retrieve/pii/S2211124717313037ae974a485f413a2113503eed53cd6c53
10.1016/j.celrep.2017.09.028
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Except where otherwise noted, this item's license is described as © 2017 The Author(s). This is an open access article under the CC BY-NC-ND license.
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