Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress

Bhattacharya, Dhruva; Yu, Li; Wang, Mingwu

dc.contributor.author	Bhattacharya, Dhruva
dc.contributor.author	Yu, Li
dc.contributor.author	Wang, Mingwu
dc.date.accessioned	2017-12-05T16:06:46Z
dc.date.available	2017-12-05T16:06:46Z
dc.date.issued	2017-11-07
dc.identifier.citation	Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress 2017, 12 (11):e0187188 PLOS ONE	en
dc.identifier.issn	1932-6203
dc.identifier.pmid	29112967
dc.identifier.doi	10.1371/journal.pone.0187188
dc.identifier.uri	http://hdl.handle.net/10150/626191
dc.description.abstract	The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse conjunctiva epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.
dc.description.sponsorship	Arizona Biomedical Research Commission Research [ADHS14-082988]; Department of Ophthalmology and Vision Science at University of Arizona	en
dc.language.iso	en	en
dc.publisher	PUBLIC LIBRARY SCIENCE	en
dc.relation.url	http://dx.plos.org/10.1371/journal.pone.0187188	en
dc.rights	© 2017 Bhattacharya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.title	Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress	en
dc.type	Article	en
dc.contributor.department	Univ Arizona, Coll Med, Dept Ophthalmol & Vis Sci	en
dc.identifier.journal	PLOS ONE	en
dc.description.note	Open access journal.	en
dc.description.collectioninformation	This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.	en
dc.eprint.version	Final published version	en
refterms.dateFOA	2018-09-12T00:21:36Z
html.description.abstract	The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse conjunctiva epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.