Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD
AffiliationUniv Arizona, Coll Med, Dept Physiol
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PublisherNATURE PUBLISHING GROUP
CitationComparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD 2017, 7 (1) Scientific Reports
Rights© The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.
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AbstractAnimal model of cigarette smoke (CS) -induced chronic obstructive pulmonary disease (COPD) is the primary testing methodology for drug therapies and studies on pathogenic mechanisms of disease. However, researchers have rarely run simultaneous or side-by-side tests of whole-body and nose-only CS exposure in building their mouse models of COPD. We compared and evaluated these two different methods of CS exposure, plus airway Lipopolysaccharides (LPS) inhalation, in building our COPD mouse model. Compared with the control group, CS exposed mice showed significant increased inspiratory resistance, functional residual capacity, right ventricular hypertrophy index, and total cell count in BALF. Moreover, histological staining exhibited goblet cell hyperplasia, lung inflammation, thickening of smooth muscle layer on bronchia, and lung angiogenesis in both methods of CS exposure. Our data indicated that a viable mouse model of COPD can be established by combining the results from wholebody CS exposure, nose-only CS exposure, and airway LPS inhalation testing. However, in our study, we also found that, given the same amount of particulate intake, changes in right ventricular pressure and intimal thickening of pulmonary small artery are a little more serious in nose-only CS exposure method than changes in the whole-body CS exposure method.
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SponsorsNational Natural Science Foundation of China [81630004, 81470246, 81220108001, 81520108001]; Guangdong Department of Science and Technology of China [2016A030311020, 2016A030313606]; Guangzhou Department of Education Yangcheng Scholarship [12A001S]; Guangzhou Department of Education Scholarship ; Guangzhou Department of Science and Technology [2014Y2-00167, 201607010358]; Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme, China