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    Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness

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    Author
    Fonseca, Larissa Fernanda Simielli
    Gimenez, Daniele Fernanda Jovino
    dos Santos Silva, Danielly Beraldo
    Barthelson, Roger
    Baldi, Fernando
    Ferro, Jesus Aparecido
    Albuquerque, Lucia Galvão
    Affiliation
    Univ Arizona, CyVerse
    Issue Date
    2017-12-04
    Keywords
    RNA-Seq
    Transcriptome
    Meat quality
    
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    Publisher
    BIOMED CENTRAL LTD
    Citation
    Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness 2017, 18 (1) BMC Genomics
    Journal
    BMC Genomics
    Rights
    © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Background: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre-and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness.
    Note
    Open access journal.
    ISSN
    1471-2164
    PubMed ID
    29202705
    DOI
    10.1186/s12864-017-4323-0
    Version
    Final published version
    Sponsors
    project "Genomic tools for the genetic improvement of traits of direct economic importance in Nelore cattle"; Sao Paulo Research Foundation - FAPESP [2009/16118-5, 2013/09190-7]
    Additional Links
    https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-4323-0
    ae974a485f413a2113503eed53cd6c53
    10.1186/s12864-017-4323-0
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