Structural analyses of key features in the KANK1·KIF21A complex yield mechanistic insights into the cross-talk between microtubules and the cell cortex
AffiliationUniv Arizona, Ctr Biomed Informat & Biostat
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CitationStructural analyses of key features in the KANK1·KIF21A complex yield mechanistic insights into the cross-talk between microtubules and the cell cortex 2018, 293 (1):215 Journal of Biological Chemistry
JournalJournal of Biological Chemistry
Rights© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThe cross-talk between dynamic microtubules and the cell cortex plays important roles in cell division, polarity, and migration. A critical adaptor that links the plus ends of microtubules with the cell cortex is the KANK N-terminal motif and ankyrin repeat domains 1 (KANK1)/kinesin family member 21A (KIF21A) complex. Genetic defects in these two proteins are associated with various cancers and developmental diseases, such as congenital fibrosis of the extraocular muscles type 1. However, the molecular mechanism governing the KANK1/KIF21A interaction and the role of the conserved ankyrin (ANK) repeats in this interaction are still unclear. In this study, we present the crystal structure of the KANK1 center dot KIF21A complex at 2.1 angstrom resolution. The structure, together with biochemical studies, revealed that a five-helix-bundle-capping domain immediately preceding the ANK repeats of KANK1 forms a structural and functional supramodule with its ANK repeats in binding to an evolutionarily conserved peptide located in the middle of KIF21A. We also show that several missense mutations present in cancer patients are located at the interface of the KANK1 center dot KIF21A complex and destabilize its formation. In conclusion, our study elucidates the molecular basis underlying the KANK1/KIF21A interaction and also provides possible mechanistic explanations for the diseases caused by mutations in KANK1 and KIF21A.
Note12 month embargo; published online: 20 November 2017
VersionFinal published version
SponsorsNational Natural Science Foundation of China [31470733, U1532121]; Shanghai Municipal Science and Technology Commission, China ("Yang-Fan program") Grant [14YF1406700]; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB08030104]
CollectionsUA Faculty Publications
- Structural insights into ankyrin repeat-mediated recognition of the kinesin motor protein KIF21A by KANK1, a scaffold protein in focal adhesion.
- Authors: Pan W, Sun K, Tang K, Xiao Q, Ma C, Yu C, Wei Z
- Issue date: 2018 Feb 9
- Structural basis for the recognition of kinesin family member 21A (KIF21A) by the ankyrin domains of KANK1 and KANK2 proteins.
- Authors: Guo Q, Liao S, Zhu Z, Li Y, Li F, Xu C
- Issue date: 2018 Jan 12
- A major mutation of KIF21A associated with congenital fibrosis of the extraocular muscles type 1 (CFEOM1) enhances translocation of Kank1 to the membrane.
- Authors: Kakinuma N, Kiyama R
- Issue date: 2009 Sep 4
- Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing.
- Authors: Li CC, Kuo JC, Waterman CM, Kiyama R, Moss J, Vaughan M
- Issue date: 2011 Nov 29
- Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions.
- Authors: Bouchet BP, Gough RE, Ammon YC, van de Willige D, Post H, Jacquemet G, Altelaar AM, Heck AJ, Goult BT, Akhmanova A
- Issue date: 2016 Jul 13