Rescue of conformational dynamics in enzyme catalysis by directed evolution
Kenner, Lillian R.
Clarkson, Michael W.
Tawfik, Dan S.
Fraser, James S.
AffiliationUniv Arizona, Biol Sci West
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationOtten, R., Liu, L., Kenner, L. R., Clarkson, M. W., Mavor, D., Tawfik, D. S., ... & Fraser, J. S. (2018). Rescue of conformational dynamics in enzyme catalysis by directed evolution. Nature communications, 9(1), 1314.
Rights© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License.
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AbstractRational design and directed evolution have proved to be successful approaches to increase catalytic efficiencies of both natural and artificial enzymes. Protein dynamics is recognized as important, but due to the inherent flexibility of biological macromolecules it is often difficult to distinguish which conformational changes are directly related to function. Here, we use directed evolution on an impaired mutant of the proline isomerase CypA and identify two second-shell mutations that partially restore its catalytic activity. We show both kinetically, using NMR spectroscopy, and structurally, by room-temperature X-ray crystallography, how local perturbations propagate through a large allosteric network to facilitate conformational dynamics. The increased catalysis selected for in the evolutionary screen is correlated with an accelerated interconversion between the two catalytically essential conformational substates, which are both captured in the high-resolution X-ray ensembles. Our data provide a glimpse of an evolutionary trajectory and show how subtle changes can fine-tune enzyme function.
VersionFinal published version
SponsorsHoward Hughes Medical Institute; Office of Basic Energy Sciences; Catalysis Science Program; U.S. Dept. of Energy [DE-FG02-05ER15699]; NIH [GM100966, GM110580]; Kinship Foundation; Pew Charitable Trusts; David and Lucile Packard Foundation; NSF [STC-1231306]; Damon Runyon Cancer Research Foundation [DRG-2114-12]; Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy [DE-AC02-05CH11231]; UC Office of the President, Multicampus Research Programs and Initiatives [MR-15-32859]; Program Breakthrough Biomedical Research - Sandler Foundation