Repeat associated mechanisms of genome evolution and function revealed by the Mus caroli and Mus pahari genomes
Navarro, Fábio C.P.
Karn, Robert C.
Laukaitis, Christina M.
Quail, Michael A.
Van Oosterhout, Cock
Adams, David J.
Keane, Thomas M.
Odom, Duncan T.
AffiliationUniv Arizona, Coll Med, Dept Med
MetadataShow full item record
CitationThybert, D., Roller, M., Navarro, F. C., Fiddes, I., Streeter, I., Feig, C., ... & Aken, B. (2018). Repeat associated mechanisms of genome evolution and function revealed by the Mus caroli and Mus pahari genomes. Genome research, 28(4), 448-459.
Rights© 2018 Thybert et al. This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International).
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AbstractUnderstanding the mechanisms driving lineage-specific evolution in both primates and rodents has been hindered by the lack of sister clades with a similar phylogenetic structure having high-quality genome assemblies. Here, we have created chromosome-level assemblies of the Mus caroli and Mus pahari genomes. Together with the Mus musculus and Rattus norvegicus genomes, this set of rodent genomes is similar in divergence times to the Hominidae (human-chimpanzee-gorilla-orangutan). By comparing the evolutionary dynamics between the Muridae and Hominidae, we identified punctate events of chromosome reshuffling that shaped the ancestral karyotype of Mus musculus and Mus caroli between 3 and 6 million yr ago, but that are absent in the Hominidae. Hominidae show between four- and sevenfold lower rates of nucleotide change and feature turnover in both neutral and functional sequences, suggesting an underlying coherence to the Muridae acceleration. Our system of matched, high-quality genome assemblies revealed how specific classes of repeats can play lineage-specific roles in related species. Recent LINE activity has remodeled protein-coding loci to a greater extent across the Muridae than the Hominidae, with functional consequences at the species level such as reproductive isolation. Furthermore, we charted a Muridae-specific retrotransposon expansion at unprecedented resolution, revealing how a single nucleotide mutation transformed a specific SINE element into an active CTCF binding site carrier specifically in Mus caroli, which resulted in thousands of novel, species-specific CTCF binding sites. Our results show that the comparison of matched phylogenetic sets of genomes will be an increasingly powerful strategy for understanding mammalian biology.
Note6 month embargo; published online: 21 March 2018
VersionFinal published version
SponsorsWellcome Trust [WT108749/Z/15/Z, WT098051, WT202878/Z/16/Z, WT202878/B/16/Z]; National Human Genome Research Institute [U41HG007234]; Cancer Research UK ; European Research Council ; Biotechnology and Biological Sciences Research Council [BB/N02317X/a]; European Molecular Biology Laboratory; European Community's Seventh Framework Programme (FP7) ; European Union's Seventh Framework Programme (FP7) [HEALTH-F4-2010-241504]
- CENP-B binds a novel centromeric sequence in the Asian mouse Mus caroli.
- Authors: Kipling D, Mitchell AR, Masumoto H, Wilson HE, Nicol L, Cooke HJ
- Issue date: 1995 Aug
- L1 gene conversion or same-site transposition.
- Authors: Burton FH, Loeb DD, Edgell MH, Hutchison CA 3rd
- Issue date: 1991 Sep
- Functional respiratory chain analyses in murid xenomitochondrial cybrids expose coevolutionary constraints of cytochrome b and nuclear subunits of complex III.
- Authors: McKenzie M, Chiotis M, Pinkert CA, Trounce IA
- Issue date: 2003 Jul
- Praomys tullbergi (Muridae, Rodentia) genome architecture decoded by comparative chromosome painting with Mus and Rattus.
- Authors: Chaves R, Louzada S, Meles S, Wienberg J, Adega F
- Issue date: 2012 Aug
- Use of repetitive DNA sequences to distinguish Mus musculus and Mus caroli cells by in situ hybridization.
- Authors: Siracusa LD, Chapman VM, Bennett KL, Hastie ND, Pietras DF, Rossant J
- Issue date: 1983 Feb