November 20, 2018: Most content in the UA Campus Repository is not accessible using the search/browse functions due to a performance bug; we are actively working to resolve this issue. If you are looking for content you know is in the repository, but cannot get to it, please email us at email@example.com with your questions and we'll make sure to get the content to you.
An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly
AuthorMcLamarrah, Tiffany A.
Buster, Daniel W.
Galletta, Brian J.
Boese, Cody J.
Ryniawec, John M.
Hollingsworth, Natalie Ann
Byrnes, Amy E.
Brownlee, Christopher W.
Slep, Kevin C.
Rusan, Nasser M.
Rogers, Gregory C.
AffiliationUniv Arizona, Canc Ctr, Dept Cellular & Mol Med
MetadataShow full item record
PublisherROCKEFELLER UNIV PRESS
CitationAn ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly Tiffany A. McLamarrah, Daniel W. Buster, Brian J. Galletta, Cody J. Boese, John M. Ryniawec, Natalie Ann Hollingsworth, Amy E. Byrnes, Christopher W. Brownlee, Kevin C. Slep, Nasser M. Rusan, Gregory C. Rogers J Cell Biol Apr 2018, 217 (4) 1217-1231; DOI: 10.1083/jcb.201605106
JournalJOURNAL OF CELL BIOLOGY
Rights© 2018 McLamarrah et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractPolo-like kinase 4 (Plk4) initiates an early step in centriole assembly by phosphorylating Ana2/STIL, a structural component of the procentriole. Here, we show that Plk4 binding to the central coiled-coil (CC) of Ana2 is a conserved event involving Polo-box 3 and a previously unidentified putative CC located adjacent to the kinase domain. Ana2 is then phosphorylated along its length. Previous studies showed that Plk4 phosphorylates the C-terminal STil/ANa2 (STAN) domain of Ana2/STIL, triggering binding and recruitment of the cartwheel protein Sas6 to the procentriole assembly site. However, the physiological relevance of N-terminal phosphorylation was unknown. We found that Plk4 first phosphorylates the extreme N terminus of Ana2, which is critical for subsequent STAN domain modification. Phosphorylation of the central region then breaks the Plk4-Ana2 interaction. This phosphorylation pattern is important for centriole assembly and integrity because replacement of endogenous Ana2 with phospho-Ana2 mutants disrupts distinct steps in Ana2 function and inhibits centriole duplication.
Note6 month embargo; published online: 1 March 2018
VersionFinal published version
SponsorsDivision of Intramural Research at the National Heart, Lung, and Blood Institute [1ZIA HL006104]; National Cancer Institute [P30 CA23074]; National Institute of General Medical Sciences [R01GFM110166]; National Science Foundation [MCB1158151]; Phoenix Friends
- Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation.
- Authors: Dzhindzhev NS, Tzolovsky G, Lipinszki Z, Abdelaziz M, Debski J, Dadlez M, Glover DM
- Issue date: 2017 Dec
- Plk4-dependent phosphorylation of STIL is required for centriole duplication.
- Authors: Kratz AS, Bärenz F, Richter KT, Hoffmann I
- Issue date: 2015 Feb 20
- Plk4 phosphorylates Ana2 to trigger Sas6 recruitment and procentriole formation.
- Authors: Dzhindzhev NS, Tzolovsky G, Lipinszki Z, Schneider S, Lattao R, Fu J, Debski J, Dadlez M, Glover DM
- Issue date: 2014 Nov 3
- A key centriole assembly interaction interface between human PLK4 and STIL appears to not be conserved in flies.
- Authors: Cottee MA, Johnson S, Raff JW, Lea SM
- Issue date: 2017 Mar 15
- Bimodal Binding of STIL to Plk4 Controls Proper Centriole Copy Number.
- Authors: Ohta M, Watanabe K, Ashikawa T, Nozaki Y, Yoshiba S, Kimura A, Kitagawa D
- Issue date: 2018 Jun 12