Show simple item record

dc.contributor.authorBornelöv, Susanne
dc.contributor.authorSeroussi, Eyal
dc.contributor.authorYosefi, Sara
dc.contributor.authorBenjamini, Sharon
dc.contributor.authorMiyara, Shoval
dc.contributor.authorRuzal, Mark
dc.contributor.authorGrabherr, Manfred
dc.contributor.authorRafati, Nima
dc.contributor.authorMolin, Anna-Maja
dc.contributor.authorPendavis, Ken
dc.contributor.authorBurgess, Shane C.
dc.contributor.authorAndersson, Leif
dc.contributor.authorFriedman-Einat, Miriam
dc.date.accessioned2018-05-23T16:07:30Z
dc.date.available2018-05-23T16:07:30Z
dc.date.issued2018-04-26
dc.identifier.citationBornelöv, S., Seroussi, E., Yosefi, S., Benjamini, S., Miyara, S., Ruzal, M., ... & Burgess, S. C. (2018). Comparative omics and feeding manipulations in chicken indicate a shift of the endocrine role of visceral fat towards reproduction. BMC genomics, 19(1), 295.en_US
dc.identifier.issn1471-2164
dc.identifier.pmid29695257
dc.identifier.doi10.1186/s12864-018-4675-0
dc.identifier.urihttp://hdl.handle.net/10150/627758
dc.description.abstractBackground: The mammalian adipose tissue plays a central role in energy-balance control, whereas the avian visceral fat hardly expresses leptin, the key adipokine in mammals. Therefore, to assess the endocrine role of adipose tissue in birds, we compared the transcriptome and proteome between two metabolically different types of chickens, broilers and layers, bred towards efficient meat and egg production, respectively. Results: Broilers and layer hens, grown up to sexual maturation under free-feeding conditions, differed 4.0-fold in weight and 1.6-fold in ovarian-follicle counts, yet the relative accumulation of visceral fat was comparable. RNA-seq and mass-spectrometry (MS) analyses of visceral fat revealed differentially expressed genes between broilers and layers, 1106 at the mRNA level (FDR = 0.05), and 203 at the protein level (P = 0.05). In broilers, Ingenuity Pathway Analysis revealed activation of the PTEN-pathway, and in layers increased response to external signals. The expression pattern of genes encoding fatsecreted proteins in broilers and layers was characterized in the RNA-seq andMS data, as well as by qPCR on visceral fat under free feeding and 24 h-feed deprivation. This characterization was expanded using available RNA-seq data of tissues from red junglefowl, and of visceral fat from broilers of different types. These comparisons revealed expression of new adipokines and secreted proteins (LCAT, LECT2, SERPINE2, SFTP1, ZP1, ZP3, APOV1, VTG1 and VTG2) at the mRNA and/or protein levels, with dynamic gene expression patterns in the selected chicken lines (except for ZP1; FDR/P = 0.05) and feed deprivation (NAMPT, SFTPA1 and ZP3) (P <= 0.05). In contrast, some of the most prominent adipokines in mammals, leptin, TNF, IFNG, and IL6 were expressed at a low level (FPKM/RPKM<1) and did not show differential mRNA expression neither between broiler and layer lines nor between fed vs. feed-deprived chickens. Conclusions: Our study revealed that RNA and protein expression in visceral fat changes with selective breeding, suggesting endocrine roles of visceral fat in the selected phenotypes. In comparison to gene expression in visceral fat of mammals, our findings points to a more direct cross talk of the chicken visceral fat with the reproductive system and lower involvement in the regulation of appetite, inflammation and insulin resistance.en_US
dc.description.sponsorshipIsrael Academy of Sciences [876/14, 1294/17]; Israeli Ministry of Agriculture [0469/14]en_US
dc.language.isoenen_US
dc.publisherBIOMED CENTRAL LTDen_US
dc.relation.urlhttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4675-0en_US
dc.rights© The Author(s). 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.en_US
dc.subjectChickensen_US
dc.subjectAdipose tissueen_US
dc.subjectAdipokinesen_US
dc.subjectPTEN-pathwayen_US
dc.subjectAdipolinen_US
dc.subjectSFTPA1en_US
dc.subjectTNFen_US
dc.subjectPLIN1en_US
dc.subjectYolk proteinsen_US
dc.subjectRNA-seqen_US
dc.subjectMass spectrometryen_US
dc.titleComparative omics and feeding manipulations in chicken indicate a shift of the endocrine role of visceral fat towards reproductionen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Coll Agr & Life Scien_US
dc.identifier.journalBMC GENOMICSen_US
dc.description.noteOpen access journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleBMC Genomics
dc.source.volume19
dc.source.issue1
refterms.dateFOA2018-05-23T16:07:30Z


Files in this item

Thumbnail
Name:
s12864-018-4675-0.pdf
Size:
1.996Mb
Format:
PDF
Description:
Final Published version

This item appears in the following Collection(s)

Show simple item record