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    A field strain of minute virus of mice (MVMm) exhibits age- and strain-specific pathogenesis

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    MVMmManuscript_FINAL_2.28.2018.pdf
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    Description:
    Final Accepted Manuscript
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    Author
    Brownlee, Rachel D.
    Ardeshir, Amir
    Becker, Michael D.
    Wagner, April M.
    Besselsen, David G.
    Affiliation
    Univ Arizona, Univ Anim Care
    Issue Date
    2018-04
    Keywords
    pathogenesis
    parvovirus
    minute virus of mice
    
    Metadata
    Show full item record
    Publisher
    MICROBIOLOGY SOC
    Citation
    Brownlee, R. D., Ardeshir, A., Becker, M. D., Wagner, A. M., & Besselsen, D. G. (2018). A field strain of minute virus of mice (MVMm) exhibits age-and strain-specific pathogenesis. Journal of General Virology.
    Journal
    JOURNAL OF GENERAL VIROLOGY
    Rights
    © 2018 The Authors. Published by the Microbiology Society.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    The influence of mouse strain, immune competence and age on the pathogenesis of a field strain of minute virus of mice (MVMm) was examined in BALB/c, C3H, C57BL/6 and SCID mice experimentally infected as neonates, weanlings and adults. Sera, bodily excretions and tissues were harvested at 7, 14, 28 and 56 days after inoculation and evaluated by serology, quantitative PCR and histopathology. Seroconversion to recombinant viral capsid protein 2 was consistently observed in all immunocompetent strains of mice, regardless of the age at which they were inoculated, while seroconversion to the viral nonstructural protein 1 was only consistently detected in neonate inoculates. Viral DNA was detected by quantitative PCR in multiple tissues of immunocompetent mice at each time point after inoculation, with the highest levels being observed in neonate inoculates at 7 days after inoculation. In contrast, viral DNA levels in tissues and bodily excretions increased consistently over time in immunodeficient SCID mice, regardless of the age at which they were inoculated, with mortality being observed in neonatal inoculates between 28 and 56 days after inoculation. Overall, productive infection was observed more frequently in immunocompetent mice inoculated as neonates as compared to those inoculated as weanlings or adults, and immunodeficient SCID mice developed persistent, progressive infection, with mortality being observed in mice inoculated as neonates. Importantly, the clinical syndrome observed in experimentally infected SCID neonatal mice recapitulates the clinical presentation reported for the naturally infected immunodeficient NOD mu-chain knockout mice from which MVMm was initially isolated.
    Note
    12 month embargo; published online: 08 March 2018
    ISSN
    0022-1317
    1465-2099
    PubMed ID
    29517477
    DOI
    10.1099/jgv.0.001044
    Version
    Final accepted manuscript
    Sponsors
    National Center for Research Resources [R01 RR 18488-01]
    Additional Links
    http://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001044
    ae974a485f413a2113503eed53cd6c53
    10.1099/jgv.0.001044
    Scopus Count
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    UA Faculty Publications

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