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dc.contributor.authorBrownlee, Rachel D.
dc.contributor.authorArdeshir, Amir
dc.contributor.authorBecker, Michael D.
dc.contributor.authorWagner, April M.
dc.contributor.authorBesselsen, David G.
dc.date.accessioned2018-05-29T21:08:51Z
dc.date.available2018-05-29T21:08:51Z
dc.date.issued2018-04
dc.identifier.citationBrownlee, R. D., Ardeshir, A., Becker, M. D., Wagner, A. M., & Besselsen, D. G. (2018). A field strain of minute virus of mice (MVMm) exhibits age-and strain-specific pathogenesis. Journal of General Virology.en_US
dc.identifier.issn0022-1317
dc.identifier.issn1465-2099
dc.identifier.pmid29517477
dc.identifier.doi10.1099/jgv.0.001044
dc.identifier.urihttp://hdl.handle.net/10150/627823
dc.description.abstractThe influence of mouse strain, immune competence and age on the pathogenesis of a field strain of minute virus of mice (MVMm) was examined in BALB/c, C3H, C57BL/6 and SCID mice experimentally infected as neonates, weanlings and adults. Sera, bodily excretions and tissues were harvested at 7, 14, 28 and 56 days after inoculation and evaluated by serology, quantitative PCR and histopathology. Seroconversion to recombinant viral capsid protein 2 was consistently observed in all immunocompetent strains of mice, regardless of the age at which they were inoculated, while seroconversion to the viral nonstructural protein 1 was only consistently detected in neonate inoculates. Viral DNA was detected by quantitative PCR in multiple tissues of immunocompetent mice at each time point after inoculation, with the highest levels being observed in neonate inoculates at 7 days after inoculation. In contrast, viral DNA levels in tissues and bodily excretions increased consistently over time in immunodeficient SCID mice, regardless of the age at which they were inoculated, with mortality being observed in neonatal inoculates between 28 and 56 days after inoculation. Overall, productive infection was observed more frequently in immunocompetent mice inoculated as neonates as compared to those inoculated as weanlings or adults, and immunodeficient SCID mice developed persistent, progressive infection, with mortality being observed in mice inoculated as neonates. Importantly, the clinical syndrome observed in experimentally infected SCID neonatal mice recapitulates the clinical presentation reported for the naturally infected immunodeficient NOD mu-chain knockout mice from which MVMm was initially isolated.en_US
dc.description.sponsorshipNational Center for Research Resources [R01 RR 18488-01]en_US
dc.language.isoenen_US
dc.publisherMICROBIOLOGY SOCen_US
dc.relation.urlhttp://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001044en_US
dc.rights© 2018 The Authors | Published by the Microbiology Societyen_US
dc.subjectpathogenesisen_US
dc.subjectparvovirusen_US
dc.subjectminute virus of miceen_US
dc.titleA field strain of minute virus of mice (MVMm) exhibits age- and strain-specific pathogenesisen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Univ Anim Careen_US
dc.identifier.journalJOURNAL OF GENERAL VIROLOGYen_US
dc.description.note12 month embargo; published online: 08 March 2018en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleJournal of General Virology
dc.source.volume99
dc.source.issue4
dc.source.beginpage558
dc.source.endpage566


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