μPAD Fluorescence Scattering Immunoagglutination Assay for Cancer Biomarkers from Blood and Serum
AffiliationUniv Arizona, Dept Biomed Engn
carcinoembryonic antigen (CEA)
carbohydrate antigen 19-9 (CA 19-9)
MetadataShow full item record
PublisherSAGE PUBLICATIONS INC
CitationBaynes, C., & Yoon, J. Y. (2017). μPAD Fluorescence Scattering Immunoagglutination Assay for Cancer Biomarkers from Blood and Serum. SLAS TECHNOLOGY: Translating Life Sciences Innovation, Vol 23, Issue 1, pp. 30 - 43, https://doi.org/10.1177%2F2472630317731891
RightsCopyright © 2018, © SAGE Publications
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractA microfluidic paper analytical device (PAD) was created for the sensitive quantification of cancer antigens, carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9), from human whole blood and serum, toward diagnosis and prognosis of colorectal cancer. Anti-CEA and anti-CA 19-9 antibodies were covalently linked to submicron, fluorescent polystyrene particles, loaded, and then dried in the center of the PAD channel. CEA- or CA 19-9-spiked blood or serum samples were loaded to the inlet of PAD, and subsequent immunoagglutination changed the fluorescent scatter signals upon ultraviolet (UV) excitation. The total assay time was about 1 min. Detection limits were 1 pg/mL for CEA and 0.1 U/mL for CA 19-9 from both 10% diluted blood and undiluted serum. The use of UV excitation and subsequent fluorescence scattering enabled much higher double-normalized intensities (up to 1.28-3.51, compared with 1.067 with the elastic Mie scatter detection), successful detection in the presence of blood or serum, and distinct multiplex assays with minimum cross-reaction of antibodies. The results with undiluted serum showed the larger dynamic range and smaller standard errors, which can be attributed to the presence of serum proteins, functioning as a stabilizer or a passivating protein for the particles within paper fibers.
VersionFinal accepted manuscript
SponsorsUA/NASA Space Grant Graduate Fellowship; BIO5 Institute at the University of Arizona
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