Validation of salivary oxytocin and vasopressin as biomarkers in domestic dogs
AffiliationUniv Arizona, Sch Anthropol
MetadataShow full item record
PublisherELSEVIER SCIENCE BV
CitationE.L. MacLean, L.R. Gesquiere, N. Gee, K. Levy, W.L. Martin, C.S. Carter Validation of salivary oxytocin and vasopressin as biomarkers in domestic dogs J. Neurosci. Methods, 293 (2018), pp. 67-76
JournalJOURNAL OF NEUROSCIENCE METHODS
Rights© 2017 Elsevier B.V. All rights reserved.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractBackground: Oxytocin (OT) and Vasopressin (AVP) are phylogenetically conserved neuropeptides with effects on social behavior, cognition and stress responses. Although OT and AVP are-most commonly measured in blood, urine and cerebrospinal fluid (CSF), these approaches present an array of challenges including concerns related to the invasiveness of sample collection, the potential for matrix interference in immunoassays, and whether samples can be collected at precise time points to assess event-linked endocrine responses. New method: We validated enzyme-linked immunosorbent assays (ELISAs) for the measurement of salivary OT and AVP in domestic dogs. Results: Both OT and AVP were present in dog saliva and detectable by ELISA and high performance liquid chromatography - mass spectrometry (HPLC-MS). OT concentrations in dog saliva were much higher than those typically detected in humans. OT concentrations in the same samples analyzed with and without sample extraction were highly correlated, but this was not true for AVP. ELISA validation studies revealed good accuracy and parallelism, both with and without solid phase extraction. Collection of salivary samples with different synthetic swabs, or following salivary stimulation or the consumption of food led to variance in results. However, samples collected from the same dogs using different techniques tended to be positively correlated. We detected concurrent elevations in salivary and plasma OT during nursing. Comparison with existing methods: There are currently no other validated methods for measuring OT/AVP in dog saliva. Conclusions: OT and AVP are present in dog saliva, and ELISAs for their detection are methodologically valid. (C) 2017 Elsevier B.V. All rights reserved.
Note18 month embargo; published online: 1 September 2017
VersionFinal accepted manuscript
SponsorsWALTHAM Centre for Pet Nutrition
- Effects of Affiliative Human-Animal Interaction on Dog Salivary and Plasma Oxytocin and Vasopressin.
- Authors: MacLean EL, Gesquiere LR, Gee NR, Levy K, Martin WL, Carter CS
- Issue date: 2017
- Development and Validation of a Simple LC-MS Method for the Quantification of Oxytocin in Dog Saliva.
- Authors: Wang L, Marti DW, Anderson RE
- Issue date: 2019 Aug 24
- Salivary Oxytocin and Vasopressin Levels in Police Officers With and Without Post-Traumatic Stress Disorder.
- Authors: Frijling JL, van Zuiden M, Nawijn L, Koch SB, Neumann ID, Veltman DJ, Olff M
- Issue date: 2015 Oct
- (Re-)activity in the caregiving situation: Genetic diversity within Oxytocin-Vasopressin Pathway is associated with salivary oxytocin and vasopressin concentrations in response to contact with a crying infant-simulator.
- Authors: Rybicka M, Kaźmierczak M, Pawlicka P, Łada-Maśko AB, Anikiej-Wiczenbach P, Bielawski KP
- Issue date: 2021 Sep
- Endogenous Oxytocin, Vasopressin, and Aggression in Domestic Dogs.
- Authors: MacLean EL, Gesquiere LR, Gruen ME, Sherman BL, Martin WL, Carter CS
- Issue date: 2017