A Capillary Flow Dynamics-Based Sensing Modality for Direct Environmental Pathogen Monitoring
AffiliationUniv Arizona, Dept Agr & Biosyst Engn
Univ Arizona, Mel & Enid Zuckerman Coll Publ Hlth
Univ Arizona, Dept Biomed Engn
MetadataShow full item record
PublisherWILEY-V C H VERLAG GMBH
CitationK. E. Klug, K. A. Reynolds, J.-Y. Yoon, Chem. Eur. J. 2018, 24, 6025.
JournalCHEMISTRY-A EUROPEAN JOURNAL
Rights© 2018 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractToward ultra-simple and field-ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody-conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper-based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target-particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring.
Note12 month embargo; published online: 05 February 2018
VersionFinal accepted manuscript
SponsorsBIO5 Institute, The University of Arizona; U.S. National Science Foundation (NSF) Water, Environment and Technology (WET) Center [IIP-1361815]; Tucson Water; NSF [DGE-1143953]