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dc.contributor.advisorMaggert, Keith
dc.contributor.authorRay, Julianne Florence
dc.creatorRay, Julianne Florence
dc.date.accessioned2018-06-25T20:57:58Z
dc.date.available2018-06-25T20:57:58Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/10150/628079
dc.description.abstractNucleosome modifications in the genome influence local heterochromatin state. Varied examples of epigenetics rely on transgenerational effects and heritability of expression states between cell (mitotic) or organismal (meiotic) generations. Most of the current focus is on transgenerational inheritance of expression states determined by methylation of lysine-4 and lysine-9 sites of histone H3. We will use photoswitchable protein Dendra2 fused to histone protein H3 to test the nature of histone-based heritability in the germline stem cell niche of male Drosophila melanogaster. Photoswitching provides a solid time-point measure of which H3 proteins are present at the time of UV exposure and which proteins are new at the time of photography. This will provide an opportunity to monitor chromosome-scale histone stability, recycling, and turnover, to monitor chromosome movement in live nuclei, and to critically test histone inheritance through cell division. Photoswitching allows us to generate a snapshot of histone behavior and elucidate the contribution of expression state modification to transgenerational effects. Here, we present background, recent progress in creation and future experimentation.en_US
dc.language.isoen_USen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjectAsymmetric inheritanceen_US
dc.subjectChromatinen_US
dc.subjectDrosophilaen_US
dc.subjectGermline stem cellen_US
dc.subjectH3en_US
dc.subjectHistoneen_US
dc.titleUsing Photoswitchable Proteins to Assess Chromatin Stabilityen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelmastersen_US
dc.contributor.committeememberWeinert, Ted
dc.contributor.committeememberThorne, Curtis
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineCellular and Molecular Medicineen_US
thesis.degree.nameM.S.en_US
refterms.dateFOA2018-06-25T20:57:58Z


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