Show simple item record

dc.contributor.advisorViswanathan, V.K.
dc.contributor.authorMonasky, Ross Calvin*
dc.creatorMonasky, Ross Calvin
dc.date.accessioned2018-06-27T23:22:13Z
dc.date.available2018-06-27T23:22:13Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/10150/628172
dc.description.abstractEnteropathogenic Escherichia coli (EPEC) is a leading cause of infantile diarrhea, particularly in developing countries. EPEC belongs to the attaching and effacing (A/E) family of pathogens and harbors a type III secretion system (T3SS) that delivers virulence proteins directly into host epithelial cells. These proteins alter host structure and function, likely facilitating pathogenesis. We recently demonstrated that EspH, an EPEC secreted protein, is a critical virulence factor and that mutant strains lacking espH are impaired for pathogenesis. EspH induces host cell death through activation of caspases and mitochondrial fission. We hypothesizes that a wide range of host proteins are implicated in this cell death phenotype. Quantitation of host cell death during EPEC infection using siRNA-mediated knockdown of individual host cell proteins supports this hypothesis. A broad group of host protein knockdowns displayed altered host cell death during infection. The goal of my studies is to identify the host pathway(s) altered during EspH-induced epithelial cell death and, eventually, to establish the significance of this pathway in EPEC virulence.en_US
dc.language.isoen_USen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjectApoptosisen_US
dc.subjectEPECen_US
dc.subjectsiRNAen_US
dc.titleThe Role of EspH and Host Cell Proteins in Enteropathogenic Escherichia Coli-Induced Cell Death and Virulenceen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelmastersen_US
dc.contributor.committeememberVedantam, Gayatri
dc.contributor.committeememberCapaldi, Andrew
dc.description.releaseRelease after 30-Apr-2024en_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMicrobiologyen_US
thesis.degree.nameM.S.en_US
dc.description.admin-noteOriginally set to release after 10-May-2020; contacted by author on 03-May-2019 to extend embargo through 30-Apr-2024, Kimberly


Files in this item

Thumbnail
Name:
azu_etd_16315_sip1_m.pdf
Embargo:
2024-04-30
Size:
1.583Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record