Molecular diversity and allergenic profiles of Alternaria spp. from desert environments in Arizona
Final Accepted Manuscript
AffiliationUniv Arizona, Coll Agr, Sch Plant Sci
MetadataShow full item record
PublisherELSEVIER SCI LTD
CitationRotondo, F., Hong, S. G., Peever, T., & Pryor, B. M. (2018). Molecular diversity and allergenic profiles of Alternaria spp. from desert environments in Arizona. Fungal biology, 122(1), 74-85, https://doi.org/10.1016/j.funbio.2017.10.010
Rights© 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThis study examined the genetic diversity of small-spored Alternaria species in the southwest desert of the USA by sampling 552 isolates from different habitats (soil and plant debris) in different locations (urban and an undisturbed desert). To estimate the genetic diversity, Amplified Fragment Length Polymorphism (AFLP) fingerprinting analysis was performed for all isolates. Strains representative of the sampled genotypic diversity (n = 125) were further characterized according their sporulation pattern and the capability to produce allergens. Morphological characterization assigned the majority of the strains to the Alternaria alternata and Alternaria tenuissima morpho-groups with only two isolates assigned to the Alternaria arborescens morpho-group. AFLP fingerprinting differentiated the A. arborescens morpho-groups, but could not distinguish between the A. altemata and A. tenuissima morpho-groups. Western blot analysis showed that a large number of allergenic proteins were produced by strains. These proteins were not specific for any morpho-group nor source of isolation. A hierarchical analysis of molecular variance was performed on the AFLP data to quantify molecular variation and partition this variation among sampled locations and habitat. No statistically significant differentiation among locations and habitat was detected indicating a lack of population structure across environments. (C) 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Note12 month embargo; published online: 1 November 2017
VersionFinal accepted manuscript
SponsorsUniversity of Arizona College of Agriculture and Life Sciences; Washington State University College of Agricultural, Human, and Natural Resource Sciences; Arizona Disease Control Research Council (ADCRC) ; National Science Foundation (DEB) 
- Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat.
- Authors: Oviedo MS, Sturm ME, Reynoso MM, Chulze SN, Ramirez ML
- Issue date: 2013
- Polyphasic classification of Alternaria isolated from hazelnut and walnut fruit in Europe.
- Authors: Hong SG, Maccaroni M, Figuli PJ, Pryor BM, Belisario A
- Issue date: 2006 Nov
- AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree.
- Authors: Somma S, Pose G, Pardo A, Mulè G, Pinto VF, Moretti A, Logrieco AF
- Issue date: 2011 Feb 28
- Comparison of Alternaria spp. collected in Italy from apple with A. mali and other AM-toxin producing strains.
- Authors: Rotondo F, Collina M, Brunelli A, Pryor BM
- Issue date: 2012 Dec
- Discrete lineages within Alternaria alternata species group: Identification using new highly variable loci and support from morphological characters.
- Authors: Armitage AD, Barbara DJ, Harrison RJ, Lane CR, Sreenivasaprasad S, Woodhall JW, Clarkson JP
- Issue date: 2015 Nov