Sensitive and specific post-call filtering of genetic variants in xenograft and primary tumors
AffiliationUniv Arizona, Dept Epidemiol & Biostat, Mel & Enid Zuckerman Coll Publ Hlth
Univ Arizona, Ctr Canc
Univ Arizona, Dept Med
Univ Arizona, Dept Pathol
Univ Arizona, Dept Mol & Cellular Biol
MetadataShow full item record
PublisherOXFORD UNIV PRESS
CitationBrian K Mannakee, Uthra Balaji, Agnieszka K Witkiewicz, Ryan N Gutenkunst, Erik S Knudsen; Sensitive and specific post-call filtering of genetic variants in xenograft and primary tumors, Bioinformatics, Volume 34, Issue 10, 15 May 2018, Pages 1713–1718, https://doi.org/10.1093/bioinformatics/bty010
Rights© The Author(s) 2018. Published by Oxford University Press. All rights reserved.
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AbstractMotivation: Tumor genome sequencing offers great promise for guiding research and therapy, but spurious variant calls can arise from multiple sources. Mouse contamination can generate many spurious calls when sequencing patient-derived xenografts. Paralogous genome sequences can also generate spurious calls when sequencing any tumor. We developed a BLAST-based algorithm, Mouse And Paralog EXterminator (MAPEX), to identify and filter out spurious calls from both these sources. Results: When calling variants from xenografts, MAPEX has similar sensitivity and specificity to more complex algorithms. When applied to any tumor, MAPEX also automatically flags calls that potentially arise from paralogous sequences. Our implementation, mapexr, runs quickly and easily on a desktop computer. MAPEX is thus a useful addition to almost any pipeline for calling genetic variants in tumors.
Note12 month embargo; published online: 08 January 2018
VersionFinal accepted manuscript
SponsorsNational Science Foundation [DGE-1143953]; National Institutes of Health [R01CA211878-01, P30CA023074-36S2]