Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa
AuthorGrover, Jeffrey W.
King, Graham J.
Mosher, Rebecca A.
AffiliationUniv Arizona, Dept Mol & Cellular Biol
Univ Arizona, Sch Plant Sci
MetadataShow full item record
CitationGrover, J. W., Kendall, T. , Baten, A. , Burgess, D. , Freeling, M. , King, G. J. and Mosher, R. A. (2018), Maternal components of RNA‐directed DNA methylation are required for seed development in Brassica rapa. Plant J, 94: 575-582. doi:10.1111/tpj.13910
Rights© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd
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AbstractSmall RNAs trigger repressive DNA methylation at thousands of transposable elements in a process called RNA-directed DNA methylation (RdDM). The molecular mechanism of RdDM is well characterized in Arabidopsis, yet the biological function remains unclear, as loss of RdDM in Arabidopsis causes no overt defects, even after generations of inbreeding. It is known that 24 nucleotide Pol IV-dependent siRNAs, the hallmark of RdDM, are abundant in flowers and developing seeds, indicating that RdDM might be important during reproduction. Here we show that, unlike Arabidopsis, mutations in the Pol IV-dependent small RNA pathway cause severe and specific reproductive defects in Brassica rapa. High rates of abortion occur when seeds have RdDM mutant mothers, but not when they have mutant fathers. Although abortion occurs after fertilization, RdDM function is required in maternal somatic tissue, not in the female gametophyte or the developing zygote, suggesting that siRNAs from the maternal soma might function in filial tissues. We propose that recently outbreeding species such as B.rapa are key to understanding the role of RdDM during plant reproduction.
Note12 month embargo; published online: 23 March 2018
VersionFinal accepted manuscript
SponsorsNSF Plant Genome Research Program [IOS-1546825]
- Arabidopsis RNA Polymerases IV and V Are Required To Establish H3K9 Methylation, but Not Cytosine Methylation, on Geminivirus Chromatin.
- Authors: Jackel JN, Storer JM, Coursey T, Bisaro DM
- Issue date: 2016 Aug 15
- DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV.
- Authors: Zhang H, Ma ZY, Zeng L, Tanaka K, Zhang CJ, Ma J, Bai G, Wang P, Zhang SW, Liu ZW, Cai T, Tang K, Liu R, Shi X, He XJ, Zhu JK
- Issue date: 2013 May 14
- NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation.
- Authors: He XJ, Hsu YF, Pontes O, Zhu J, Lu J, Bressan RA, Pikaard C, Wang CS, Zhu JK
- Issue date: 2009 Feb 1
- Full-length autonomous transposable elements are preferentially targeted by expression-dependent forms of RNA-directed DNA methylation.
- Authors: Panda K, Ji L, Neumann DA, Daron J, Schmitz RJ, Slotkin RK
- Issue date: 2016 Aug 9
- Maize RNA PolIV affects the expression of genes with nearby TE insertions and has a genome-wide repressive impact on transcription.
- Authors: Forestan C, Farinati S, Aiese Cigliano R, Lunardon A, Sanseverino W, Varotto S
- Issue date: 2017 Oct 12