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dc.contributor.authorGao, Meijing
dc.contributor.authorWang, Ximeng
dc.contributor.authorYang, Yihua
dc.contributor.authorTabashnik, Bruce E.
dc.contributor.authorWu, Yidong
dc.date.accessioned2018-07-24T23:32:37Z
dc.date.available2018-07-24T23:32:37Z
dc.date.issued2018-06
dc.identifier.citationGao M, Wang X, Yang Y, Tabashnik BE, Wu Y. Epistasis confers resistance to Bt toxin Cry1Ac in the cotton bollworm. Evol Appl. 2018;11:809–819. https://doi.org/10.1111/eva.12598en_US
dc.identifier.issn17524571
dc.identifier.doi10.1111/eva.12598
dc.identifier.urihttp://hdl.handle.net/10150/628295
dc.description.abstractEvolution of resistance by insect pests reduces the benefits of extensively cultivated transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Previous work showed that resistance to Bt toxin Cry1Ac, which is produced by transgenic cotton, can be conferred by mutations disrupting a cadherin protein that binds this Bt toxin in the larval midgut. However, the potential for epistatic interactions between the cadherin gene and other genes has received little attention. Here, we report evidence of epistasis conferring resistance to Cry1Ac in the cotton bollworm, Helicoverpa armigera, one of the world's most devastating crop pests. Resistance to Cry1Ac in strain LF256 originated from a field-captured male and was autosomal, recessive, and 220-fold relative to susceptible strain SCD. We conducted complementation tests for allelism by crossing LF256 with a strain in which resistance to Cry1Ac is conferred by a recessive allele at the cadherin locus HaCad. The resulting F-1 offspring were resistant, suggesting that resistance to Cry1Ac in LF256 is also conferred by resistance alleles at this locus. However, the HaCad amino acid sequence in LF256 lacked insertions and deletions, and did not differ consistently between LF256 and a susceptible strain. In addition, most of the cadherin alleles in LF256 were not derived from the field-captured male. Moreover, Cry1Ac resistance was not genetically linked with the HaCad locus in LF256. Furthermore, LF256 and the susceptible strain were similar in levels of HaCad transcript, cadherin protein, and binding of Cry1Ac to cadherin. Overall, the results imply that epistasis between HaCad and an unknown second locus in LF256 yielded the observed resistance in the F-1 progeny from the complementation test. The observed epistasis has important implications for interpreting results of the F-1 screen used widely to monitor and analyze resistance, as well as the potential to accelerate evolution of resistance.en_US
dc.description.sponsorshipNational Natural Science Foundation of China [31530060]; Ministry of Agriculture of China [2016ZX08012-004]; Innovation Team Program for Jiangsu universities [2013-6]; U.S. Department of Agriculture [2014-33522-22214]en_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.urlhttp://doi.wiley.com/10.1111/eva.2018.11.issue-5en_US
dc.relation.urlhttp://doi.wiley.com/10.1111/eva.12598en_US
dc.rights© 2018 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License.en_US
dc.subjectallelismen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectcotton bollwormen_US
dc.subjectF-1 screenen_US
dc.subjectgenetically engineered cropen_US
dc.subjectresistance managementen_US
dc.subjectsecond-site noncomplementationen_US
dc.subjecttransgenic cottonen_US
dc.titleEpistasis confers resistance to Bt toxin Cry1Ac in the cotton bollwormen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Entomolen_US
dc.identifier.journalEVOLUTIONARY APPLICATIONSen_US
dc.description.noteOpen Access Journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleEvolutionary Applications
dc.source.volume11
dc.source.issue5
dc.source.beginpage809
dc.source.endpage819
refterms.dateFOA2018-07-24T23:32:38Z


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