Huang, Yvonne J.
Christian, Laura S.
Mark Ansel, K.
Martin, Richard J.
Mauger, David T.
Rosenberg, Sharon R.
King, Tonya S.
White, Steven R.
Denlinger, Loren C.
Lazarus, Stephen C.
Peters, Stephen P.
Smith, Lewis J.
Wechsler, Michael E.
Lynch, Susan V.
Boushey, Homer A.
AffiliationUniv Arizona, Hlth Sci Ctr
Induced sputum microbiota
MetadataShow full item record
PublisherBIOMED CENTRAL LTD
CitationDurack, J., Huang, Y. J., Nariya, S., Christian, L. S., Ansel, K. M., Beigelman, A., ... & Martin, R. J. (2018). Bacterial biogeography of adult airways in atopic asthma. Microbiome, 6(1), 104. https://doi.org/10.1186/s40168-018-0487-3
Rights© The Author(s). 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractBackground: Perturbations to the composition and function of bronchial bacterial communities appear to contribute to the pathophysiology of asthma. Unraveling the nature and mechanisms of these complex associations will require large longitudinal studies, for which bronchoscopy is poorly suited. Studies of samples obtained by sputum induction and nasopharyngeal brushing or lavage have also reported asthma-associated microbiota characteristics. It remains unknown, however, whether the microbiota detected in these less-invasive sample types reflect the composition of bronchial microbiota in asthma. Results: Bacterial microbiota in paired protected bronchial brushings (BB; n = 45), induced sputum (IS; n = 45), oral wash (OW; n = 45), and nasal brushings (NB; n = 27) from adults with mild atopic asthma (AA), atopy without asthma (ANA), and healthy controls (HC) were profiled using 16S rRNA gene sequencing. Though microbiota composition varied with sample type (p < 0.001), compositional similarity was greatest for BB-IS, particularly in AAs and ANAs. The abundance of genera detected in BB correlated with those detected in IS and OW (r median [IQR] 0.869 [0.748-0.942] and 0.822 [0.687-0.909] respectively), but not with those in NB (r = 0.004 [-0.003-0.011]). The number of taxa shared between IS-BB and NB-BB was greater in AAs than in HCs (p < 0.05) and included taxa previously associated with asthma. Of the genera abundant in NB, only Moraxella correlated positively with abundance in BB; specific members of this genus were shared between the two compartments only in AAs. Relative abundance of Moraxella in NB of AAs correlated negatively with that of Corynebacterium but positively with markers of eosinophilic inflammation in the blood and BAL fluid. The genus, Corynebacterium, trended to dominate all NB samples of HCs but only half of AAs (p = 0.07), in whom abundance of this genus was negatively associated with markers of eosinophilic inflammation. Conclusions: Induced sputum is superior to nasal brush or oral wash for assessing bronchial microbiota composition in asthmatic adults. Although compositionally similar to the bronchial microbiota, the microbiota in induced sputum are distinct, reflecting enrichment of oral bacteria. Specific bacterial genera are shared between the nasal and the bronchial mucosa which are associated with markers of systemic and bronchial inflammation.
VersionFinal published version
SponsorsNational Heart, Lung, and Blood Institute (NHLBI)/AsthmaNet [HL098107]; National Institute of Allergy and Infectious Disease (NIAID)/Inner City Asthma Consortium (ICAC) [AI114271]; NIAID [1R01AI129958]; NHLBI [1R03HL138310]
- Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment.
- Authors: Durack J, Lynch SV, Nariya S, Bhakta NR, Beigelman A, Castro M, Dyer AM, Israel E, Kraft M, Martin RJ, Mauger DT, Rosenberg SR, Sharp-King T, White SR, Woodruff PG, Avila PC, Denlinger LC, Holguin F, Lazarus SC, Lugogo N, Moore WC, Peters SP, Que L, Smith LJ, Sorkness CA, Wechsler ME, Wenzel SE, Boushey HA, Huang YJ, National Heart, Lung and Blood Institute's “AsthmaNet”.
- Issue date: 2017 Jul
- Sputum microbiota in severe asthma patients: Relationship to eosinophilic inflammation.
- Authors: Li N, Qiu R, Yang Z, Li J, Chung KF, Zhong N, Zhang Q
- Issue date: 2017 Oct
- Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways.
- Authors: Denner DR, Sangwan N, Becker JB, Hogarth DK, Oldham J, Castillo J, Sperling AI, Solway J, Naureckas ET, Gilbert JA, White SR
- Issue date: 2016 May
- The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients.
- Authors: Millares L, Bermudo G, Pérez-Brocal V, Domingo C, Garcia-Nuñez M, Pomares X, Moya A, Monsó E
- Issue date: 2017 Jan 19
- Bacterial microbiota of the upper respiratory tract and childhood asthma.
- Authors: Depner M, Ege MJ, Cox MJ, Dwyer S, Walker AW, Birzele LT, Genuneit J, Horak E, Braun-Fahrländer C, Danielewicz H, Maier RM, Moffatt MF, Cookson WO, Heederik D, von Mutius E, Legatzki A
- Issue date: 2017 Mar