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    Global deletion of tetraspanin CD82 attenuates bone growth and enhances bone marrow adipogenesis

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    Global_CD82_KO_Bone_Manuscript ...
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    Final Accepted Manuscript
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    Author
    Bergsma, Alexis
    Ganguly, Sourik S.
    Dick, Daniel
    Williams, Bart O.
    Miranti, Cindy K.
    Affiliation
    Univ Arizona, Ctr Canc
    Issue Date
    2018-08
    Keywords
    Tetraspanin
    Bone remodeling
    Mouse model
    CD82
    Osteoblasts
    Adipocytes
    
    Metadata
    Show full item record
    Publisher
    ELSEVIER SCIENCE INC
    Citation
    Bergsma, A., Ganguly, S. S., Dick, D., Williams, B. O., & Miranti, C. K. (2018). Global deletion of tetraspanin CD82 attenuates bone growth and enhances bone marrow adipogenesis. Bone, 113, 105-113. https://doi.org/10.1016/j.bone.2018.05.020
    Journal
    BONE
    Rights
    © 2018 Elsevier Inc. All rights reserved.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    CD82 is a widely expressed member of the tetraspanin family of transmembrane proteins known to control cell signaling, adhesion, and migration. Tetraspanin CD82 is induced over 9-fold during osteoclast differentiation in vitro; however, its role in bone homeostasis is unknown. A globally deleted CD82 mouse model was used to assess the bone phenotype. Based on microCT and 4-point bending tests, CD82-deficient bones are smaller in diameter and weaker, but display no changes in bone density. Histomorphometry shows a decrease in size, erosion perimeter, and number of osteoclasts in situ, with a corresponding increase in trabecular surface area, specifically in male mice. Male-specific alterations are observed in trabecular structure by microCT and in vitro differentiated osteoclasts are morphologically abnormal. Histomorphometry did not reveal a significant reduction in osteoblast number; however, dynamic labeling reveals a significant decrease in bone growth. Consistent with defects in OB function, OB differentiation and mineralization are defective in vitro, whereas adipogenesis is enhanced. There is a corresponding increase in bone marrow adipocytes in situ. Thus, combined defects in both osteoclasts and osteoblasts can account for the observed bone phenotypes, and suggests a role for CD82 in both bone mesenchyme and myeloid cells.
    Note
    12 month embargo; published online: 18 May 2018
    ISSN
    87563282
    PubMed ID
    29782939
    DOI
    10.1016/j.bone.2018.05.020
    Version
    Final accepted manuscript
    Sponsors
    Van Andel Research Institute; Van Andel Institute Graduate School
    Additional Links
    https://linkinghub.elsevier.com/retrieve/pii/S8756328218302102
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bone.2018.05.020
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