Show simple item record

dc.contributor.authorBergsma, Alexis
dc.contributor.authorGanguly, Sourik S.
dc.contributor.authorDick, Daniel
dc.contributor.authorWilliams, Bart O.
dc.contributor.authorMiranti, Cindy K.
dc.date.accessioned2018-08-14T23:37:29Z
dc.date.available2018-08-14T23:37:29Z
dc.date.issued2018-08
dc.identifier.citationBergsma, A., Ganguly, S. S., Dick, D., Williams, B. O., & Miranti, C. K. (2018). Global deletion of tetraspanin CD82 attenuates bone growth and enhances bone marrow adipogenesis. Bone, 113, 105-113. https://doi.org/10.1016/j.bone.2018.05.020en_US
dc.identifier.issn87563282
dc.identifier.pmid29782939
dc.identifier.doi10.1016/j.bone.2018.05.020
dc.identifier.urihttp://hdl.handle.net/10150/628516
dc.description.abstractCD82 is a widely expressed member of the tetraspanin family of transmembrane proteins known to control cell signaling, adhesion, and migration. Tetraspanin CD82 is induced over 9-fold during osteoclast differentiation in vitro; however, its role in bone homeostasis is unknown. A globally deleted CD82 mouse model was used to assess the bone phenotype. Based on microCT and 4-point bending tests, CD82-deficient bones are smaller in diameter and weaker, but display no changes in bone density. Histomorphometry shows a decrease in size, erosion perimeter, and number of osteoclasts in situ, with a corresponding increase in trabecular surface area, specifically in male mice. Male-specific alterations are observed in trabecular structure by microCT and in vitro differentiated osteoclasts are morphologically abnormal. Histomorphometry did not reveal a significant reduction in osteoblast number; however, dynamic labeling reveals a significant decrease in bone growth. Consistent with defects in OB function, OB differentiation and mineralization are defective in vitro, whereas adipogenesis is enhanced. There is a corresponding increase in bone marrow adipocytes in situ. Thus, combined defects in both osteoclasts and osteoblasts can account for the observed bone phenotypes, and suggests a role for CD82 in both bone mesenchyme and myeloid cells.en_US
dc.description.sponsorshipVan Andel Research Institute; Van Andel Institute Graduate Schoolen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S8756328218302102en_US
dc.rights© 2018 Elsevier Inc. All rights reserved.en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectTetraspaninen_US
dc.subjectBone remodelingen_US
dc.subjectMouse modelen_US
dc.subjectCD82en_US
dc.subjectOsteoblastsen_US
dc.subjectAdipocytesen_US
dc.titleGlobal deletion of tetraspanin CD82 attenuates bone growth and enhances bone marrow adipogenesisen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Ctr Cancen_US
dc.identifier.journalBONEen_US
dc.description.note12 month embargo; published online: 18 May 2018en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleBone
dc.source.volume113
dc.source.beginpage105
dc.source.endpage113


Files in this item

Thumbnail
Name:
Global_CD82_KO_Bone_Manuscript ...
Size:
7.142Mb
Format:
PDF
Description:
Final Accepted Manuscript

This item appears in the following Collection(s)

Show simple item record