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dc.contributor.authorBeyene, Getu
dc.contributor.authorSolomon, Felix R.
dc.contributor.authorChauhan, Raj D.
dc.contributor.authorGaitán-Solis, Eliana
dc.contributor.authorNarayanan, Narayanan
dc.contributor.authorGehan, Jackson
dc.contributor.authorSiritunga, Dimuth
dc.contributor.authorStevens, Robyn L.
dc.contributor.authorJifon, John
dc.contributor.authorVan Eck, Joyce
dc.contributor.authorLinsler, Edward
dc.contributor.authorGehan, Malia
dc.contributor.authorIlyas, Muhammad
dc.contributor.authorFregene, Martin
dc.contributor.authorSayre, Richard T.
dc.contributor.authorAnderson, Paul
dc.contributor.authorTaylor, Nigel J.
dc.contributor.authorCahoon, Edgar B.
dc.date.accessioned2018-08-16T18:03:58Z
dc.date.available2018-08-16T18:03:58Z
dc.date.issued2018-06
dc.identifier.citationBeyene, G. , Solomon, F. R., Chauhan, R. D., Gaitán‐Solis, E. , Narayanan, N. , Gehan, J. , Siritunga, D. , Stevens, R. L., Jifon, J. , Van Eck, J. , Linsler, E. , Gehan, M. , Ilyas, M. , Fregene, M. , Sayre, R. T., Anderson, P. , Taylor, N. J. and Cahoon, E. B. (2018), Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch. Plant Biotechnol J, 16: 1186-1200. doi:10.1111/pbi.12862en_US
dc.identifier.issn14677644
dc.identifier.pmid29193665
dc.identifier.doi10.1111/pbi.12862
dc.identifier.urihttp://hdl.handle.net/10150/628556
dc.description.abstractStorage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub-Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A beta-carotene. In this study, beta-carotene concentrations in cassava storage roots were enhanced by co-expression of transgenes for deoxy-D-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin-type 1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to <= 50 mu g/g DW, 15- to 20-fold increases relative to roots from nontransgenic plants. Approximately 85%-90% of these carotenoids accumulated as all-trans-beta-carotene, the most nutritionally efficacious carotenoid. beta-Carotene-accumulating storage roots displayed delayed onset of postharvest physiological deterioration, a major constraint limiting utilization of cassava products. Large metabolite changes were detected in beta-carotene-enhanced storage roots. Most significantly, an inverse correlation was observed between beta-carotene and dry matter content, with reductions of 50%-60% of dry matter content in the highest carotenoid-accumulating storage roots of different cultivars. Further analysis confirmed a concomitant reduction in starch content and increased levels of total fatty acids, triacylglycerols, soluble sugars and abscisic acid. Potato engineered to co-express DXS and crtB displayed a similar correlation between b-carotene accumulation, reduced dry matter and starch content and elevated oil and soluble sugars in tubers. Transcriptome analyses revealed a reduced expression of genes involved in starch biosynthesis including ADP-glucose pyrophosphorylase genes in transgenic, carotene-accumulating cassava roots relative to nontransgenic roots. These findings highlight unintended metabolic consequences of provitamin A biofortification of starch-rich organs and point to strategies for redirecting metabolic flux to restore starch production.en_US
dc.description.sponsorshipBill and Melinda Gates Foundation [OPPGD1484]; USDA-Agriculture and Food Research Initiative [2015-67013-22839]; NSF Major Research Instrumentation Grant [DBI-1427621]en_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.urlhttp://doi.wiley.com/10.1111/pbi.2018.16.issue-6en_US
dc.relation.urlhttp://doi.wiley.com/10.1111/pbi.12862en_US
dc.rights© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcassavaen_US
dc.subjectdry matteren_US
dc.subjectfatty aciden_US
dc.subjectstarchen_US
dc.subjectbeta-caroteneen_US
dc.subjectprovitamin Aen_US
dc.titleProvitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starchen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizonaen_US
dc.identifier.journalPLANT BIOTECHNOLOGY JOURNALen_US
dc.description.noteOpen Access Journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitlePlant Biotechnology Journal
dc.source.volume16
dc.source.issue6
dc.source.beginpage1186
dc.source.endpage1200
refterms.dateFOA2018-08-16T18:03:58Z


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© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License.
Except where otherwise noted, this item's license is described as © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License.