The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA
AffiliationUniv Arizona, Div Community & Syst Hlth Sci, Coll Nursing
MetadataShow full item record
PublisherTAYLOR & FRANCIS LTD
CitationAndrea M. Quinn, Allison R. Williams, Teresa I. Sivilli, Charles L. Raison & Thaddeus W. W. Pace (2018) The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA, Stress, 21:4, 376-381, DOI: 10.1080/10253890.2018.1446518
RightsRights managed by Taylor & Francis
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractCirculating concentrations of interleukin (IL)-6, an inflammatory biomarker widely assessed in humans to study the inflammatory response to acute psychological stress, have for decades been quantified using enzyme-linked immunosorbent assay (ELISA). However, biobehavioral researchers are increasingly using cytokine multiplex assays instead of ELISA to measure IL-6 and other cytokines. Despite this trend, multiplex assays have not been directly compared to ELISA for their ability to detect subtle stress-induced changes of IL-6. Here, we tested the prediction that a high-sensitivity multiplex assay (human Magnetic Luminex Performance Assay, R&D Systems, Minneapolis, MN) would detect changes in IL-6 as a result of acute stress challenge in a manner comparable to high-sensitivity ELISA. Blood was collected from 12 healthy adults immediately before and then 90 and 210min after the start of the Trier Social Stress Test (TSST), an acute laboratory psychosocial stress challenge. In addition to quantifying IL-6 concentrations in plasma with both multiplex and ELISA, we also assessed concentrations of tumor necrosis factor-alpha, IL-8, IL-10, IL-5, and IL-2 with multiplex. The multiplex detected IL-6 in all samples. Concentrations strongly correlated with values determined by ELISA across all samples (r=0.941, p<.001) as well as among samples collected at individual TSST time points. IL-6 responses to the TSST (i.e. area under the curve) captured by multiplex and ELISA were also strongly correlated (r(s)=0.937, p<.001). While other cytokines were detected by multiplex, none changed as a result of TSST challenge at time points examined. These results suggest high-sensitivity magnetic multiplex assay is able to detect changes in plasma concentrations of IL-6 as a result of acute stress in humans.
Note12 month embargo; published online: 13 March 2018
VersionFinal accepted manuscript
SponsorsNCATS NIH HHS [UL1 TR000454]; NCCIH NIH HHS [R01 AT004698]
- Simultaneous measurement of multiple ear proteins with multiplex ELISA assays.
- Authors: Trune DR, Larrain BE, Hausman FA, Kempton JB, MacArthur CJ
- Issue date: 2011 May
- Acute psychosocial stress induces differential short-term changes in catecholamine sensitivity of stimulated inflammatory cytokine production.
- Authors: Strahler J, Rohleder N, Wolf JM
- Issue date: 2015 Jan
- Stress-induced changes in LPS-induced pro-inflammatory cytokine production in chronic fatigue syndrome.
- Authors: Gaab J, Rohleder N, Heitz V, Engert V, Schad T, Schürmeyer TH, Ehlert U
- Issue date: 2005 Feb
- LPS-stimulated tumor necrosis factor-alpha and interleukin-6 mRNA and cytokine responses following acute psychological stress.
- Authors: Huang CJ, Stewart JK, Franco RL, Evans RK, Lee ZP, Cruz TD, Webb HE, Acevedo EO
- Issue date: 2011 Nov
- Single molecule measurements of tumor necrosis factor α and interleukin-6 in the plasma of patients with Crohn's disease.
- Authors: Song L, Hanlon DW, Chang L, Provuncher GK, Kan CW, Campbell TG, Fournier DR, Ferrell EP, Rivnak AJ, Pink BA, Minnehan KA, Patel PP, Wilson DH, Till MA, Faubion WA, Duffy DC
- Issue date: 2011 Sep 30