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dc.contributor.authorQuinn, Andrea M.
dc.contributor.authorWilliams, Allison R.
dc.contributor.authorSivilli, Teresa I.
dc.contributor.authorRaison, Charles L.
dc.contributor.authorPace, Thaddeus W. W.
dc.date.accessioned2018-11-02T18:04:56Z
dc.date.available2018-11-02T18:04:56Z
dc.date.issued2018
dc.identifier.citationAndrea M. Quinn, Allison R. Williams, Teresa I. Sivilli, Charles L. Raison & Thaddeus W. W. Pace (2018) The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA, Stress, 21:4, 376-381, DOI: 10.1080/10253890.2018.1446518en_US
dc.identifier.issn1025-3890
dc.identifier.issn1607-8888
dc.identifier.pmid29529950
dc.identifier.doi10.1080/10253890.2018.1446518
dc.identifier.urihttp://hdl.handle.net/10150/630575
dc.description.abstractCirculating concentrations of interleukin (IL)-6, an inflammatory biomarker widely assessed in humans to study the inflammatory response to acute psychological stress, have for decades been quantified using enzyme-linked immunosorbent assay (ELISA). However, biobehavioral researchers are increasingly using cytokine multiplex assays instead of ELISA to measure IL-6 and other cytokines. Despite this trend, multiplex assays have not been directly compared to ELISA for their ability to detect subtle stress-induced changes of IL-6. Here, we tested the prediction that a high-sensitivity multiplex assay (human Magnetic Luminex Performance Assay, R&D Systems, Minneapolis, MN) would detect changes in IL-6 as a result of acute stress challenge in a manner comparable to high-sensitivity ELISA. Blood was collected from 12 healthy adults immediately before and then 90 and 210min after the start of the Trier Social Stress Test (TSST), an acute laboratory psychosocial stress challenge. In addition to quantifying IL-6 concentrations in plasma with both multiplex and ELISA, we also assessed concentrations of tumor necrosis factor-alpha, IL-8, IL-10, IL-5, and IL-2 with multiplex. The multiplex detected IL-6 in all samples. Concentrations strongly correlated with values determined by ELISA across all samples (r=0.941, p<.001) as well as among samples collected at individual TSST time points. IL-6 responses to the TSST (i.e. area under the curve) captured by multiplex and ELISA were also strongly correlated (r(s)=0.937, p<.001). While other cytokines were detected by multiplex, none changed as a result of TSST challenge at time points examined. These results suggest high-sensitivity magnetic multiplex assay is able to detect changes in plasma concentrations of IL-6 as a result of acute stress in humans.en_US
dc.description.sponsorshipNCATS NIH HHS [UL1 TR000454]; NCCIH NIH HHS [R01 AT004698]en_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.urlhttps://www.tandfonline.com/doi/full/10.1080/10253890.2018.1446518en_US
dc.rightsRights managed by Taylor & Francisen_US
dc.subjectAcute stressen_US
dc.subjectinterleukin-6en_US
dc.subjectmultiplexen_US
dc.subjectTSSTen_US
dc.subjectELISAen_US
dc.subjecthumanen_US
dc.titleThe plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISAen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Div Community & Syst Hlth Sci, Coll Nursingen_US
dc.identifier.journalSTRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESSen_US
dc.description.note12 month embargo; published online: 13 March 2018en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleStress
dc.source.volume21
dc.source.issue4
dc.source.beginpage376
dc.source.endpage381


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