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dc.contributor.authorChamcheu, Jean
dc.contributor.authorRady, Islam
dc.contributor.authorChamcheu, Roxane-Cherille
dc.contributor.authorSiddique, Abu
dc.contributor.authorBloch, Melissa
dc.contributor.authorBanang Mbeumi, Sergette
dc.contributor.authorBabatunde, Abiola
dc.contributor.authorUddin, Mohammad
dc.contributor.authorNoubissi, Felicite
dc.contributor.authorJurutka, Peter
dc.contributor.authorLiu, Yong-Yu
dc.contributor.authorSpiegelman, Vladimir
dc.contributor.authorWhitfield, G.
dc.contributor.authorEl Sayed, Khalid
dc.date.accessioned2018-11-26T23:26:07Z
dc.date.available2018-11-26T23:26:07Z
dc.date.issued2018-06
dc.identifier.citationChamcheu JC, Rady I, Chamcheu R-CN, Siddique AB, Bloch MB, Banang Mbeumi S, Babatunde AS, Uddin MB, Noubissi FK, Jurutka PW, Liu Y-Y, Spiegelman VS, Whitfield GK, El Sayed KA. Graviola (Annona muricata) Exerts Anti-Proliferative, Anti-Clonogenic and Pro-Apoptotic Effects in Human Non-Melanoma Skin Cancer UW-BCC1 and A431 Cells In Vitro: Involvement of Hedgehog Signaling. International Journal of Molecular Sciences. 2018; 19(6):1791.en_US
dc.identifier.issn1422-0067
dc.identifier.pmid29914183
dc.identifier.doi10.3390/ijms19061791
dc.identifier.urihttp://hdl.handle.net/10150/631063
dc.description.abstractNon-melanoma skin cancers (NMSCs) are the leading cause of skin cancer-related morbidity and mortality. Effective strategies are needed to control NMSC occurrence and progression. Non-toxic, plant-derived extracts have been shown to exert multiple anti-cancer effects. Graviola (Annona muricata), a tropical fruit-bearing plant, has been used in traditional medicine against multiple human diseases including cancer. The current study investigated the effects of graviola leaf and stem extract (GLSE) and its solvent-extracted fractions on two human NMSC cell lines, UW-BCC1 and A431. GLSE was found to: (i) dose-dependently suppress UW-BCC1 and A431 cell growth, motility, wound closure, and clonogenicity; (ii) induce G(0)/G(1) cell cycle arrest by downregulating cyclin/cdk factors while upregulating cdk inhibitors, and (iii) induce apoptosis as evidenced by cleavage of caspases-3, -8 and PARP. Further, GLSE suppressed levels of activated hedgehog (Hh) pathway components Smo, Gli 1/2, and Shh while inducing SuFu. GLSE also decreased the expression of pro-apoptotic protein Bax while decreasing the expression of the anti-apoptotic protein Bcl-2. We determined that these activities were concentrated in an acetogenin/alkaloid-rich dichloromethane subfraction of GLSE. Our data identify graviola extracts and their constituents as promising sources for new chemopreventive and therapeutic agent(s) to be further developed for the control of NMSCs.en_US
dc.description.sponsorshipUniversity of Louisiana at Monroe (ULM), School of Pharmacy; ULM College of Pharmacy Faculty Research Seed Grant [5CALHN-260615]; American Skin Association (ASA); UW-Madison Skin Disease Research Center (SDRC) Pilot and Feasibility Research Award from NIH/NIAMS [P30 AR066524]; POHOFI Inc. Pre-College Intern Scholarship Award; NIH [NIH R15CA16747, R01CA140285]; University of Arizona, Department of Basic Medical Sciencesen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.urlhttp://www.mdpi.com/1422-0067/19/6/1791en_US
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectAnnona muricataen_US
dc.subjectapoptosisen_US
dc.subjectbasal cell carcinomaen_US
dc.subjectcutaneous squamous cell carcinomaen_US
dc.subjectgraviolaen_US
dc.subjectHedgehog signaling pathwayen_US
dc.subjectnatural products chemistryen_US
dc.subjectnon-melanoma skin canceren_US
dc.titleGraviola (Annona muricata) Exerts Anti-Proliferative, Anti-Clonogenic and Pro-Apoptotic Effects in Human Non-Melanoma Skin Cancer UW-BCC1 and A431 Cells In Vitro: Involvement of Hedgehog Signalingen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Coll Med Phoenix, Dept Basic Med Scien_US
dc.identifier.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCESen_US
dc.description.noteOpen Access Journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleInternational Journal of Molecular Sciences
dc.source.volume19
dc.source.issue6
dc.source.beginpage1791
refterms.dateFOA2018-11-26T23:26:08Z


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