Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters
Gerba, Charles P.
AffiliationUniv Arizona, Dept Soil Water & Environm Sci
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationHata, Akihiko & Kitajima, Masaaki & Haramoto, Eiji & Lee, Suntae & Ihara, Masaru & Gerba, Charles & Tanaka, Hiroaki. (2018). Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters. Scientific Reports. 8. 10.1038/s41598-018-30217-y.
Rights© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractHuman astroviruses are associated with gastroenteritis and known to contaminate water environments. Three different genetic clades of astroviruses are known to infect humans and each Glade consists of diverse strains. This study aimed to determine the occurrence and genetic diversity of astrovirus strains in water samples in different geographical locations, i.e., influent and effluent wastewater samples (n = 24 each) in Arizona, U.S., and groundwater (n = 37) and river water (n = 14) samples collected in the Kathmandu Valley, Nepal, using next-generation amplicon sequencing. Astrovirus strains including rare types (types 6 and 7 classical human astroviruses), emerging type (type 5 VA-astroviruses), and putative recombinants were identified. Feline astrovirus strains were collaterally identified and recombination between human and feline astroviruses was suggested. Classicaland VA-astroviruses seemed to be prevalent during cooler months, while MLB-astroviruses were identified only during warmer months. This study demonstrated the effectiveness of next-generation amplicon sequencing for identification and characterization of genetically diverse astrovirus strains in environmental water.
NoteOpen access journal.
VersionFinal published version
SponsorsJSPS [14J03643]; JSPS KAKENHI from Japan Society for the Promotion of Science [JP 26820222, JP 26289182, 17H03332]
- Wastewater Analysis Indicates that Genetically Diverse Astroviruses, Including Strains Belonging to Novel Clades MLB and VA, Are Circulating within Japanese Populations.
- Authors: Hata A, Katayama H, Kitajima M, Furumai H
- Issue date: 2015 Aug
- Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan.
- Authors: Khamrin P, Thongprachum A, Okitsu S, Hayakawa S, Maneekarn N, Ushijima H
- Issue date: 2016 Feb
- Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011-2016.
- Authors: Kumthip K, Khamrin P, Ushijima H, Maneekarn N
- Issue date: 2018 Nov
- Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats.
- Authors: Brussel KV, Wang X, Shi M, Carrai M, Li J, Martella V, Beatty JA, Holmes EC, Barrs VR
- Issue date: 2020 Nov 12
- Human stool contains a previously unrecognized diversity of novel astroviruses.
- Authors: Finkbeiner SR, Holtz LR, Jiang Y, Rajendran P, Franz CJ, Zhao G, Kang G, Wang D
- Issue date: 2009 Oct 8