Enteropathogenic Escherichia coli EspH-Mediated Rho GTPase Inhibition Results in Desmosomal Perturbations
AuthorRoxas, Jennifer Lising
Monasky, Ross Calvin
Roxas, Bryan Angelo P.
Agellon, Al B.
Kaper, James B.
AffiliationUniv Arizona, Sch Anim & Comparat Biomed Sci
Univ Arizona, BIO5 Inst Collaborat Res
Univ Arizona, Dept Immunobiol
MetadataShow full item record
CitationJennifer Lising Roxas, Ross Calvin Monasky, Bryan Angelo P. Roxas, Al B. Agellon, Asad Mansoor, James B. Kaper, Gayatri Vedantam, V.K. Viswanathan, Enteropathogenic Escherichia coli EspH-Mediated Rho GTPase Inhibition Results in Desmosomal Perturbations, Cellular and Molecular Gastroenterology and Hepatology, 6(2), 2018, pp 163-180, https://doi.org/10.1016/j.jcmgh.2018.04.007.
Rights© 2018 The Authors. Published by Elsevier Inc. on behalf of the AGA Institute.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractBACKGROUND & AIMS: The diarrheagenic pathogen, enteropathogenic Escherichia coli (EPEC), uses a type III secretion system to deliver effector molecules into intestinal epithelial cells (IECs). While exploring the basis for the lateral membrane separation of EPEC-infected IECs, we observed infection-induced loss of the desmosomal cadherin desmoglein-2 (DSG2). We sought to identify the molecule(s) involved in, and delineate the mechanisms and consequences of, EPEC-induced DSG2 loss. METHODS: DSG2 abundance and localization was monitored via immunoblotting and immunofluorescence, respectively. Junctional perturbations were visualized by electron microscopy, and cell-cell adhesion was assessed using dispase assays. EspH alanine-scan mutants as well as pharmacologic agents were used to evaluate impacts on desmosomal alterations. EPEC-mediated DSG2 loss, and its impact on bacterial colonization in vivo, was assessed using a murine model. RESULTS: The secreted virulence protein EspH mediates EPEC-induced DSG2 degradation, and contributes to desmosomal perturbation, loss of cell junction integrity, and barrier disruption in infected IECs. EspH sequesters Rho guanine nucleotide exchange factors and inhibits Rho guanosine triphosphatase signaling; EspH mutants impaired for Rho guanine nucleotide exchange factor interaction failed to inhibit RhoA or deplete DSG2. Cytotoxic necrotizing factor 1, which locks Rho guanosine triphosphatase in the active state, jasplakinolide, a molecule that promotes actin polymerization, and the lysosomal inhibitor bafilomycin A, respectively, rescued infected cells from EPEC-induced DSG2 loss. Wild-type EPEC, but not an espH-deficient strain, colonizes mouse intestines robustly, widens paracellular junctions, and induces DSG2 re-localization in vivo. CONCLUSIONS: Our studies define the mechanism and consequences of EPEC-induced desmosomal alterations in IECs. These perturbations contribute to the colonization and virulence of EPEC, and likely related pathogens. (Cell Mol Gastroenterol Hepatol 2018;6:163-180; https:/doi.org/10.1016/j.jcmgh.2018.04.007)
NoteOpen access journal.
VersionFinal published version
SponsorsNational Institutes of Health [NIAID1R01AI081742, 1S10OD011981-01]; United States Department of Agriculture Co-op Research and Extension Services (USDA CSREES) Hatch Program [ARZT-5704100-A02-140, ARZT-570410-A-02-139]; US Department of Veterans Affairs [1I01BX001183-01]
- Epithelial maturity influences EPEC-induced desmosomal alterations.
- Authors: Roxas JL, Vedantam G, Viswanathan VK
- Issue date: 2018 Sep 5
- The interplay between the Escherichia coli Rho guanine nucleotide exchange factor effectors and the mammalian RhoGEF inhibitor EspH.
- Authors: Wong AR, Clements A, Raymond B, Crepin VF, Frankel G
- Issue date: 2012
- The enteropathogenic E. coli effector EspH promotes actin pedestal formation and elongation via WASP-interacting protein (WIP).
- Authors: Wong AR, Raymond B, Collins JW, Crepin VF, Frankel G
- Issue date: 2012 Jul
- Enteropathogenic Escherichia coli inhibits type I interferon- and RNase L-mediated host defense to disrupt intestinal epithelial cell barrier function.
- Authors: Long TM, Nisa S, Donnenberg MS, Hassel BA
- Issue date: 2014 Jul
- Attaching-and-Effacing Pathogens Exploit Junction Regulatory Activities of N-WASP and SNX9 to Disrupt the Intestinal Barrier.
- Authors: Garber JJ, Mallick EM, Scanlon KM, Turner JR, Donnenberg MS, Leong JM, Snapper SB
- Issue date: 2018 Mar