Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
AffiliationUniv Arizona, Dept Biosyst Engn
Univ Arizona, Dept Biomed Engn
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationKaarj, Kattika & Akarapipad, Patarajarin & Yoon, Jeong-Yeol. (2018). Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips. Scientific Reports. 8. 10.1038/s41598-018-30797-9.
Rights© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.
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AbstractThe recent Zika virus (ZIKV) outbreak has prompted the need for field-ready diagnostics that are rapid, easy-to-use, handheld, and disposable while providing extreme sensitivity and specificity. To meet this demand, we developed a wax-printed paper microfluidic chip utilizing reverse transcription loop-mediated isothermal amplification (RT-LAMP). The developed simple and sensitive ZIKV assay was demonstrated using undiluted tap water, human urine, and diluted (10%) human blood plasma. Paper type, pore size, and channel dimension of various paper microfluidic chips were investigated and optimized to ensure proper filtration of direct-use biological samples (tap water, urine, and plasma) during capillary action-driven flow. Once ZIKV RNA has flowed and reached to a detection area of the paper microfluidic chip, it was excised for the addition of an RT-LAMP mixture with a pH indicator, then placed on a hot plate at 68 degrees C. Visible color changes from successful amplification were observed in 15 minutes and quantified by smartphone imaging. The limit of detection was as low as 1 copy/mu L. The developed platform can also be used for identifying other flaviviruses, such as Chikungunya virus (CHIKV) and Dengue virus (DENV), and potentially other quickly transmitted virus pathogens, towards field-based diagnostics.
NoteOpen access journal.
VersionFinal published version
SponsorsDevelopment and Promotion of Science and Technology Talents Project (DPST) of Thailand; One District One Scholarship (ODOS) of Thailand; BIO5 Institute at the University of Arizona