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dc.contributor.authorKaarj, Kattika*
dc.contributor.authorAkarapipad, Patarajarin*
dc.contributor.authorYoon, Jeong-Yeol*
dc.date.accessioned2018-12-14T22:43:45Z
dc.date.available2018-12-14T22:43:45Z
dc.date.issued2018-08-20
dc.identifier.citationKaarj, Kattika & Akarapipad, Patarajarin & Yoon, Jeong-Yeol. (2018). Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips. Scientific Reports. 8. 10.1038/s41598-018-30797-9.en_US
dc.identifier.issn2045-2322
dc.identifier.pmid30127503
dc.identifier.doi10.1038/s41598-018-30797-9
dc.identifier.urihttp://hdl.handle.net/10150/631192
dc.description.abstractThe recent Zika virus (ZIKV) outbreak has prompted the need for field-ready diagnostics that are rapid, easy-to-use, handheld, and disposable while providing extreme sensitivity and specificity. To meet this demand, we developed a wax-printed paper microfluidic chip utilizing reverse transcription loop-mediated isothermal amplification (RT-LAMP). The developed simple and sensitive ZIKV assay was demonstrated using undiluted tap water, human urine, and diluted (10%) human blood plasma. Paper type, pore size, and channel dimension of various paper microfluidic chips were investigated and optimized to ensure proper filtration of direct-use biological samples (tap water, urine, and plasma) during capillary action-driven flow. Once ZIKV RNA has flowed and reached to a detection area of the paper microfluidic chip, it was excised for the addition of an RT-LAMP mixture with a pH indicator, then placed on a hot plate at 68 degrees C. Visible color changes from successful amplification were observed in 15 minutes and quantified by smartphone imaging. The limit of detection was as low as 1 copy/mu L. The developed platform can also be used for identifying other flaviviruses, such as Chikungunya virus (CHIKV) and Dengue virus (DENV), and potentially other quickly transmitted virus pathogens, towards field-based diagnostics.en_US
dc.description.sponsorshipDevelopment and Promotion of Science and Technology Talents Project (DPST) of Thailand; One District One Scholarship (ODOS) of Thailand; BIO5 Institute at the University of Arizonaen_US
dc.language.isoenen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.relation.urlhttp://www.nature.com/articles/s41598-018-30797-9en_US
dc.rights© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.titleSimpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chipsen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Biosyst Engnen_US
dc.contributor.departmentUniv Arizona, Dept Biomed Engnen_US
dc.identifier.journalSCIENTIFIC REPORTSen_US
dc.description.noteOpen access journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleScientific Reports
dc.source.volume8
dc.source.issue1
refterms.dateFOA2018-12-14T22:43:46Z


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