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dc.contributor.authorMaghsoudi, Kobra
dc.contributor.authorEmam, Yahya
dc.contributor.authorNiazi, Ali
dc.contributor.authorPessarakli, Mohammad
dc.contributor.authorArvin, Mohammad Javad
dc.date.accessioned2018-12-17T20:52:40Z
dc.date.available2018-12-17T20:52:40Z
dc.date.issued2018-08-09
dc.identifier.citationKobra Maghsoudi, Yahya Emam, Ali Niazi, Mohammad Pessarakli & Mohammad Javad Arvin (2018) P5CS expression level and proline accumulation in the sensitive and tolerant wheat cultivars under control and drought stress conditions in the presence/absence of silicon and salicylic acid, Journal of Plant Interactions, 13:1, 461-471, DOI: 10.1080/17429145.2018.1506516en_US
dc.identifier.issn1742-9145
dc.identifier.issn1742-9153
dc.identifier.doi10.1080/17429145.2018.1506516
dc.identifier.urihttp://hdl.handle.net/10150/631213
dc.description.abstractThe effects of silicon (Si) and salicylic acid (SA) applications on proline content and expression of (1)-pyrrolin-5-carboxylate synthetase (P5CS) were examined under different drought levels and different drought exposure times. Two wheat cultivars, a drought tolerant and a drought sensitive were used. The experiment was a factorial based on completely randomized design with three replicates. Expression analysis by the quantitative real time PCR showed that the tolerant cultivar had significantly higher P5CS expressions compared to the sensitive one under drought stress. In sampling time points, the maximum level of mRNA was observed at 48h after stress was applied. At 48h after stress induction, the expression of P5CS was almost 3.1 fold higher in the tolerant cultivar compared to the sensitive one. In both cultivars, gene expression decreased from 48 to 72h. The stressed plants treated with Si+SA showed a higher expression. Proline content started to increase by Si and SA treatments and the maximum proline content was obtained at simultaneous application of Si+SA. Drought stress significantly reduced chlorophyll content, relative water content and leaf water potential of both cultivars, while increased electrolyte leakage (EL) of the leaves. In contrast, foliar-applied Si and SA significantly increased these parameters and reduced EL, and the effect of simultaneous application of Si and SA was greater. The results suggest that the P5CS is a stress inducible gene. This gene has the potential to be used for improvement of drought stress tolerance in wheat. Network analysis highlighted positive interaction of osmotic stress, drought and cold stress on P5CS1 and the regulatory role of MYB2, ERF-1, and EIN3 transcription factors. In conclusion, alleviation of drought stress by application of Si and SA was associated partially with enhanced expression of P5CS gene and following proline accumulation.en_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.urlhttps://www.tandfonline.com/doi/full/10.1080/17429145.2018.1506516en_US
dc.rights© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.en_US
dc.subjectLeaf water potentialen_US
dc.subjectnetwork analysisen_US
dc.subjectP5CSen_US
dc.subjectreal time- PCRen_US
dc.titleP5CS expression level and proline accumulation in the sensitive and tolerant wheat cultivars under control and drought stress conditions in the presence/absence of silicon and salicylic aciden_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Plant Scien_US
dc.identifier.journalJOURNAL OF PLANT INTERACTIONSen_US
dc.description.noteOpen access journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleJournal of Plant Interactions
dc.source.volume13
dc.source.issue1
dc.source.beginpage461
dc.source.endpage471
refterms.dateFOA2018-12-17T20:52:40Z


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