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dc.contributor.authorSharaf, Radwa*
dc.contributor.authorLee, Guinevere Q*
dc.contributor.authorSun, Xiaoming*
dc.contributor.authorEtemad, Behzad*
dc.contributor.authorAboukhater, Layla M*
dc.contributor.authorHu, Zixin*
dc.contributor.authorBrumme, Zabrina L*
dc.contributor.authorAga, Evgenia*
dc.contributor.authorBosch, Ronald J*
dc.contributor.authorWen, Ying*
dc.contributor.authorNamazi, Golnaz*
dc.contributor.authorGao, Ce*
dc.contributor.authorAcosta, Edward P*
dc.contributor.authorGandhi, Rajesh T*
dc.contributor.authorJacobson, Jeffrey M*
dc.contributor.authorSkiest, Daniel*
dc.contributor.authorMargolis, David M*
dc.contributor.authorMitsuyasu, Ronald*
dc.contributor.authorVolberding, Paul*
dc.contributor.authorConnick, Elizabeth*
dc.contributor.authorKuritzkes, Daniel R*
dc.contributor.authorLederman, Michael M*
dc.contributor.authorYu, Xu G*
dc.contributor.authorLichterfeld, Mathias*
dc.contributor.authorLi, Jonathan Z*
dc.date.accessioned2019-01-03T17:39:40Z
dc.date.available2019-01-03T17:39:40Z
dc.date.issued2018-08-31
dc.identifier.citationJ Clin Invest. 2018;128(9):4074–4085. https://doi.org/10.1172/JCI120549.en_US
dc.identifier.issn1558-8238
dc.identifier.pmid30024859
dc.identifier.doi10.1172/JCI120549
dc.identifier.urihttp://hdl.handle.net/10150/631258
dc.description.abstractHIV posttreatment controllers (PTCs) represent a natural model of sustained HIV remission, but they are rare and little is known about their viral reservoir. We obtained 1,450 proviral sequences after near-full-length amplification for 10 PTCs and 16 posttreatment noncontrollers (NCs). Before treatment interruption, the median intact and total reservoir size in PTCs was 7-fold lower than in NCs, but the proportion of intact, defective, and total clonally expanded proviral genomes was not significantly different between the 2 groups. Quantification of total but not intact proviral genome copies predicted sustained HIV remission as 81% of NCs, but none of the PTCs had a total proviral genome greater than 4 copies per million peripheral blood mononuclear cells (PBMCs). The results highlight the restricted intact and defective HIV reservoir in PTCs and suggest that total proviral genome burden could act as the first biomarker for identifying PTCs. Total and defective but not intact proviral copy numbers correlated with levels of cell-associated HIV RNA, activated NK cell percentages, and both HIV-specific CD4+ and CD8+ responses. These results support the concept that defective HIV genomes can lead to viral antigen production and interact with both the innate and adaptive immune systems.en_US
dc.description.sponsorshipNational Institutes of Health/National Institute of Allergy and Infectious Diseases [AI125109]; Harvard University Center for AIDS Research [5P30AI060354-08, 5P30AI060354-14]; Michael Smith Foundation for Health Research; [UM1AI068634]; [UM1AI068636]; [UM1AI106701]; [UM1AI126617]; [UM1AI069423]en_US
dc.language.isoenen_US
dc.publisherAMER SOC CLINICAL INVESTIGATION INCen_US
dc.relation.urlhttps://www.jci.org/articles/view/120549en_US
dc.rightsCopyright © 2018, American Society for Clinical Investigationen_US
dc.subjectAIDS vaccineen_US
dc.subjectAIDS/HIVen_US
dc.subjectInfectious diseaseen_US
dc.titleHIV-1 proviral landscapes distinguish posttreatment controllers from noncontrollers.en_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Coll Meden_US
dc.identifier.journalJOURNAL OF CLINICAL INVESTIGATIONen_US
dc.description.noteOpen access journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleThe Journal of clinical investigation
refterms.dateFOA2019-01-03T17:39:41Z


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