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dc.contributor.authorLam, Wing Y.
dc.contributor.authorJash, Arijita
dc.contributor.authorYao, Cong-Hui
dc.contributor.authorD’Souza, Lucas
dc.contributor.authorWong, Rachel
dc.contributor.authorNunley, Ryan M.
dc.contributor.authorMeares, Gordon P.
dc.contributor.authorPatti, Gary J.
dc.contributor.authorBhattacharya, Deepta
dc.date.accessioned2019-01-03T19:42:53Z
dc.date.available2019-01-03T19:42:53Z
dc.date.issued2018-08-28
dc.identifier.citationWing Y. Lam, Arijita Jash, Cong-Hui Yao, Lucas D’Souza, Rachel Wong, Ryan M. Nunley, Gordon P. Meares, Gary J. Patti, Deepta Bhattacharya, Metabolic and Transcriptional Modules Independently Diversify Plasma Cell Lifespan and Function, Cell Reports, Volume 24, Issue 9, 2018, Pages 2479-2492.e6, https://doi.org/10.1016/j.celrep.2018.07.084.en_US
dc.identifier.issn22111247
dc.identifier.pmid30157439
dc.identifier.doi10.1016/j.celrep.2018.07.084
dc.identifier.urihttp://hdl.handle.net/10150/631265
dc.description.abstractPlasma cell survival and the consequent duration of immunity vary widely with infection or vaccination. Using fluorescent glucose analog uptake, we defined multiple developmentally independent mouse plasma cell populations with varying lifespans. Long-lived plasma cells imported more fluorescent glucose analog, expressed higher surface levels of the amino acid transporter CD98, and had more autophagosome mass than did short-lived cells. Low amino acid concentrations triggered reductions in both antibody secretion and mitochondrial respiration, especially by short-lived plasma cells. To explain these observations, we found that glutamine was used for both mitochondrial respiration and anaplerotic reactions, yielding glutamate and aspartate for antibody synthesis. Endoplasmic reticulum (ER) stress responses, which link metabolism to transcriptional outcomes, were similar between long- and short-lived subsets. Accordingly, population and single-cell transcriptional comparisons across mouse and human plasma cell subsets revealed few consistent and conserved differences. Thus, plasma cell antibody secretion and lifespan are primarily defined by non-transcriptional metabolic traits.en_US
dc.description.sponsorshipNIH [R01AI099108, R01AI129945, R01ES022181, R01NS099304]; New York Stem Cell Foundation; National Science Foundation [DGE-1143954]; NCI Cancer Center Support Grant [P30 CA91842]; ICTS/CTSA from the National Center for Research Resources (NCRR), a component of the NIH [UL1TR000448]; NIH Roadmap for Medical Researchen_US
dc.language.isoenen_US
dc.publisherCELL PRESSen_US
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S2211124718312026en_US
dc.rights© 2018 The Author(s).en_US
dc.titleMetabolic and Transcriptional Modules Independently Diversify Plasma Cell Lifespan and Functionen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Coll Med, Dept Immunobiolen_US
dc.identifier.journalCELL REPORTSen_US
dc.description.noteOpen access journal.en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleCell Reports
dc.source.volume24
dc.source.issue9
dc.source.beginpage2479
dc.source.endpage2492.e6
refterms.dateFOA2019-01-03T19:42:53Z


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