Microfluidic flow-based platforms for induction and analysis of dynamic shear-mediated platelet activation—Initial validation versus the standardized hemodynamic shearing device
Author
Dimasi, AnnalisaRoka-Moiia, Yana
Consolo, Filippo
Rasponi, Marco
Fiore, Gianfranco B.

Slepian, Marvin J
Redaelli, Alberto

Affiliation
Univ Arizona, Sarver Heart Ctr, Dept Med & Biomed EngnIssue Date
2018-07
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AMER INST PHYSICSCitation
Biomicrofluidics 12, 042208 (2018); https://doi.org/10.1063/1.5024500Journal
BIOMICROFLUIDICSRights
© 2018 Author(s).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
A microfluidic flow-based platform (mu FP), able to stimulate platelets via exposure of shear stress patterns pertinent to cardiovascular devices and prostheses, was compared to the Hemodynamic Shearing Device (HSD)-a state-of-the-art benchtop system for exposure of platelets to defined levels and patterns of shear. Platelets were exposed to time-varying shear stress patterns in the two systems; in detail, platelets were recirculated in the mu FP or stimulated in the HSD to replicate comparable exposure time. Shear-mediated platelet activation was evaluated via (i) the platelet activity state assay, allowing the measurement of platelet-mediated thrombin generation and associated prothrombotic tendencies, (ii) scanning electron microscopy to evaluate morphological changes of sheared platelets, and (iii) flow cytometry for the determination of platelet phosphatidylserine exposure as a marker of shear activation. The results revealed good matching and comparability between the two systems, with similar trends of platelet activation, formation of microaggregates, and analogous trends of activation marker exposure for both the HSD and microfluidic-stimulated samples. These findings support future translation of the microfluidic platform as a Point-of-Care facsimile system for the diagnosis of thrombotic risk in patients implanted with cardiovascular devices. Published by AIP Publishing.Note
12 month embargo; published online: 22 May 2018ISSN
1932-1058PubMed ID
29861819Version
Final published versionSponsors
Regione Lombardia [2016-0901]; Fondazione Cariplo [2015-1044]; University of Arizona Center for Accelerated Biomedical Innovation (ACABI); Tech Launch Arizona [UA 15-035]; Cancer Center Support Grant [CCSG-CA 023074]; Fondazione CariploAdditional Links
http://aip.scitation.org/doi/10.1063/1.5024500ae974a485f413a2113503eed53cd6c53
10.1063/1.5024500
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