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    Microfluidic flow-based platforms for induction and analysis of dynamic shear-mediated platelet activation—Initial validation versus the standardized hemodynamic shearing device

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    Author
    Dimasi, Annalisa
    Roka-Moiia, Yana
    Consolo, Filippo
    Rasponi, Marco
    Fiore, Gianfranco B. cc
    Slepian, Marvin J
    Redaelli, Alberto cc
    Affiliation
    Univ Arizona, Sarver Heart Ctr, Dept Med & Biomed Engn
    Issue Date
    2018-07
    
    Metadata
    Show full item record
    Publisher
    AMER INST PHYSICS
    Citation
    Biomicrofluidics 12, 042208 (2018); https://doi.org/10.1063/1.5024500
    Journal
    BIOMICROFLUIDICS
    Rights
    © 2018 Author(s).
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    A microfluidic flow-based platform (mu FP), able to stimulate platelets via exposure of shear stress patterns pertinent to cardiovascular devices and prostheses, was compared to the Hemodynamic Shearing Device (HSD)-a state-of-the-art benchtop system for exposure of platelets to defined levels and patterns of shear. Platelets were exposed to time-varying shear stress patterns in the two systems; in detail, platelets were recirculated in the mu FP or stimulated in the HSD to replicate comparable exposure time. Shear-mediated platelet activation was evaluated via (i) the platelet activity state assay, allowing the measurement of platelet-mediated thrombin generation and associated prothrombotic tendencies, (ii) scanning electron microscopy to evaluate morphological changes of sheared platelets, and (iii) flow cytometry for the determination of platelet phosphatidylserine exposure as a marker of shear activation. The results revealed good matching and comparability between the two systems, with similar trends of platelet activation, formation of microaggregates, and analogous trends of activation marker exposure for both the HSD and microfluidic-stimulated samples. These findings support future translation of the microfluidic platform as a Point-of-Care facsimile system for the diagnosis of thrombotic risk in patients implanted with cardiovascular devices. Published by AIP Publishing.
    Note
    12 month embargo; published online: 22 May 2018
    ISSN
    1932-1058
    PubMed ID
    29861819
    DOI
    10.1063/1.5024500
    Version
    Final published version
    Sponsors
    Regione Lombardia [2016-0901]; Fondazione Cariplo [2015-1044]; University of Arizona Center for Accelerated Biomedical Innovation (ACABI); Tech Launch Arizona [UA 15-035]; Cancer Center Support Grant [CCSG-CA 023074]; Fondazione Cariplo
    Additional Links
    http://aip.scitation.org/doi/10.1063/1.5024500
    ae974a485f413a2113503eed53cd6c53
    10.1063/1.5024500
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