Microfluidic flow-based platforms for induction and analysis of dynamic shear-mediated platelet activation—Initial validation versus the standardized hemodynamic shearing device
Fiore, Gianfranco B.
Slepian, Marvin J
AffiliationUniv Arizona, Sarver Heart Ctr, Dept Med & Biomed Engn
MetadataShow full item record
PublisherAMER INST PHYSICS
CitationBiomicrofluidics 12, 042208 (2018); https://doi.org/10.1063/1.5024500
Rights© 2018 Author(s).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractA microfluidic flow-based platform (mu FP), able to stimulate platelets via exposure of shear stress patterns pertinent to cardiovascular devices and prostheses, was compared to the Hemodynamic Shearing Device (HSD)-a state-of-the-art benchtop system for exposure of platelets to defined levels and patterns of shear. Platelets were exposed to time-varying shear stress patterns in the two systems; in detail, platelets were recirculated in the mu FP or stimulated in the HSD to replicate comparable exposure time. Shear-mediated platelet activation was evaluated via (i) the platelet activity state assay, allowing the measurement of platelet-mediated thrombin generation and associated prothrombotic tendencies, (ii) scanning electron microscopy to evaluate morphological changes of sheared platelets, and (iii) flow cytometry for the determination of platelet phosphatidylserine exposure as a marker of shear activation. The results revealed good matching and comparability between the two systems, with similar trends of platelet activation, formation of microaggregates, and analogous trends of activation marker exposure for both the HSD and microfluidic-stimulated samples. These findings support future translation of the microfluidic platform as a Point-of-Care facsimile system for the diagnosis of thrombotic risk in patients implanted with cardiovascular devices. Published by AIP Publishing.
Note12 month embargo; published online: 22 May 2018
VersionFinal published version
SponsorsRegione Lombardia [2016-0901]; Fondazione Cariplo [2015-1044]; University of Arizona Center for Accelerated Biomedical Innovation (ACABI); Tech Launch Arizona [UA 15-035]; Cancer Center Support Grant [CCSG-CA 023074]; Fondazione Cariplo