Evidence for a Unique DNA-Dependent RNA Polymerase in Cereal Crops
AffiliationUniv Arizona, Dept Mol & Cellular Biol
Univ Arizona, Sch Plant Sci
MetadataShow full item record
PublisherOXFORD UNIV PRESS
CitationJoshua T Trujillo, Arun S Seetharam, Matthew B Hufford, Mark A Beilstein, Rebecca A Mosher; Evidence for a Unique DNA-Dependent RNA Polymerase in Cereal Crops, Molecular Biology and Evolution, Volume 35, Issue 10, 1 October 2018, Pages 2454–2462, https://doi.org/10.1093/molbev/msy146
JournalMOLECULAR BIOLOGY AND EVOLUTION
Rights© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractGene duplication is an important driver for the evolution of new genes and protein functions. Duplication of DNA-dependent RNA polymerase (Pol) II subunits within plants led to the emergence of RNA Pol IV and V complexes, each of which possess unique functions necessary for RNA-directed DNA Methylation. Comprehensive identification of Pol V subunit orthologs across the monocot radiation revealed a duplication of the largest two subunits within the grasses (Poaceae), including critical cereal crops. These paralogous Pol subunits display sequence conservation within catalytic domains, but their carboxy terminal domains differ in length and character of the Ago-binding platform, suggesting unique functional interactions. Phylogenetic analysis of the catalytic region indicates positive selection on one paralog following duplication, consistent with retention via neofunctionalization. Positive selection on residue pairs that are predicted to interact between subunits suggests that paralogous subunits have evolved specific assembly partners. Additional Pol subunits as well as Pol-interacting proteins also possess grass-specific paralogs, supporting the hypothesis that a novel Pol complex with distinct function has evolved in the grass family, Poaceae.
NoteOpen access article
VersionFinal published version
SponsorsNational Science Foundation [IOS-1546825]; National Institutes of Health [T32-GM008659]