Bacterial Associates of a Gregarious Riparian Beetle With Explosive Defensive Chemistry
AffiliationUniv Arizona, Dept Entomol
Univ Arizona, Dept Entomol, Ctr Insect Sci
MetadataShow full item record
PublisherFRONTIERS MEDIA SA
CitationMcManus R, Ravenscraft A and Moore W (2018) Bacterial Associates of a Gregarious Riparian Beetle With Explosive Defensive Chemistry. Front. Microbiol. 9:2361. doi: 10.3389/fmicb.2018.02361
JournalFRONTIERS IN MICROBIOLOGY
Rights© 2018 McManus, Ravenscraft and Moore. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractBombardier beetles (Carabidae: Brachininae) are well known for their unique explosive defensive chemistry. These beetles are found in riparian corridors throughout the American Southwest, where they commonly form large diurnal multispecies aggregations in moist areas under rocks, in crevices, and in leaf litter. Using high throughput 16S amplicon sequencing, we provide the first microbiome survey of a bombardier beetle, Brachinus elongatulus, collected from two sites in Arizona. Two bacterial taxa were present in all individuals sampled: Enterococcus and Dysgonomonas. Enterococcus has been implicated in the production of fecal aggregation pheromone components, which have been shown to regulate aggregation in the German cockroach; it is possible that Enterococcus plays a similar role in Brachinus. Dysgonomonas was found in all the secretory cells of the defensive system and gut samples. Additional studies are needed to determine if these microbes play a role in these beetles' unique chemical defense. Results also show that the majority of B. elongatulus individuals collected from both sites were infected with Spiroplasma. Many Spiroplasma are intracellular, vertically transmitted insect symbionts that may manipulate host reproduction (e.g., cause male-killing) or provide resistance to nematodes and/or parasitoid wasps. Defensive protection could be especially beneficial to B. elongatulus, which are frequently parasitized by horsehair worms (Nematomorpha). In sum, findings suggest several testable hypotheses on the effects bacteria may have on bombardier beetle behavior and physiology.
NoteOpen access journal.
VersionFinal published version
SponsorsUniversity of the Arizona, Office for Research and Discovery, Faculty Seed Grants Program, Microbiome Discovery and its Influence on Beetle-Ant Symbiosis; National Science Foundation ; U.S. National Institutes of Health PERT Training Grant [K12GM000708]
- Biosynthetic origin of benzoquinones in the explosive discharge of the bombardier beetle Brachinus elongatulus.
- Authors: Attygalle AB, Xu S, Moore W, McManus R, Gill A, Will K
- Issue date: 2020 Jun 16
- Molecular phylogeny, ecology and multispecies aggregation behaviour of bombardier beetles in Arizona.
- Authors: Schaller JC, Davidowitz G, Papaj DR, Smith RL, Carrière Y, Moore W
- Issue date: 2018
- Functional anatomy of the explosive defensive system of bombardier beetles (Coleoptera, Carabidae, Brachininae).
- Authors: Di Giulio A, Muzzi M, Romani R
- Issue date: 2015 Sep
- The ant nest "bomber": Explosive defensive system of the flanged bombardier beetle Paussus favieri (Coleoptera, Carabidae).
- Authors: Muzzi M, Di Giulio A
- Issue date: 2019 May
- Defense of bombardier beetles against avian predators.
- Authors: Kojima W, Yamamoto R
- Issue date: 2020 Aug 24