Comparative Assessment of BGM and PLC/PRF/5 Cell Lines for Enteric Virus Detection in Biosolids.
AffiliationUniv Arizona, Dept Soil Water & Environm Sci
MetadataShow full item record
CitationAbd-Elmaksoud, S., Castro-del Campo, N., Gerba, C.P. et al. Food Environ Virol (2019) 11: 32. https://doi.org/10.1007/s12560-019-09366-4
JournalFOOD AND ENVIRONMENTAL VIROLOGY
Rights© Springer Science+Business Media, LLC, part of Springer Nature 2019
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThe buffalo green monkey (BGM) cell line is required for the detection of enteric viruses in biosolids through a total culturable viral assay (TCVA) by the United States Environmental Protection Agency. In the present study, BGM and PLC/PRF/5 cell lines were evaluated for TCVA and for their use in determining the incidence of adenoviruses and enteroviruses in raw sludge and Class B biosolids. Six raw sludge and 17 Class B biosolid samples were collected from 13 wastewater treatment plants from seven U.S. states. Samples were processed via organic flocculation and concentrate volumes equivalent to 4 g total solids were assayed on BGM and PLC/PRF/5 cells. Cell monolayers were observed for cytopathic effect (CPE) after two 14-days passages. Cell lysates were tested for the presence of adenoviruses and enteroviruses by PCR or RT-PCR. The PLC/PRF/5 cells detected more culturable viruses than the BGM cells by CPE (73.9% vs. 56.5%, respectively). 52% of the samples were positive for CPE using both cell lines. No viruses were detected in either cell line by PCR in flasks in which CPE was not observed. No adenoviruses were detected in 13 CPE-positive samples from BGM lysates. In contrast, of the 17 samples exhibiting CPE on PLC/PRF/5 cells, 14 were positive for adenoviruses (82.4%). In conclusion, PLC/PRF/5 cells were superior for the detection of adenoviruses in both raw sludge and Class B biosolids. Thus, the use of BGM cells alone for TCVA may underestimate the viral concentration in sludge/biosolid samples.
Note12 month embargo; published online: 23 January 2019
VersionFinal accepted manuscript
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- Authors: Chapron CD, Ballester NA, Fontaine JH, Frades CN, Margolin AB
- Issue date: 2000 Jun
- Quantification of enteric viruses, pathogen indicators, and Salmonella bacteria in class B anaerobically digested biosolids by culture and molecular methods.
- Authors: Wong K, Onan BM, Xagoraraki I
- Issue date: 2010 Oct
- Effect of detection methods on risk estimates of exposure to biosolids-associated human enteric viruses.
- Authors: Kumar A, Wong K, Xagoraraki I
- Issue date: 2012 May
- Viral pollution in the environment and in shellfish: human adenovirus detection by PCR as an index of human viruses.
- Authors: Pina S, Puig M, Lucena F, Jofre J, Girones R
- Issue date: 1998 Sep