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    Comparative Assessment of BGM and PLC/PRF/5 Cell Lines for Enteric Virus Detection in Biosolids.

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    Abd-Elmaksoud_et_al._2019-final.pdf
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    Author
    Abd-Elmaksoud, Sherif
    Castro-Del Campo, Nohelia
    Gerba, Charles P
    Pepper, Ian L
    Bright, Kelly R
    Affiliation
    Univ Arizona, Dept Soil Water & Environm Sci
    Issue Date
    2019-03-01
    Keywords
    Adenovirus
    BGM cell line
    Biosolids
    Enteric viruses
    ICC-PCR
    PLC cell line
    
    Metadata
    Show full item record
    Publisher
    SPRINGER
    Citation
    Abd-Elmaksoud, S., Castro-del Campo, N., Gerba, C.P. et al. Food Environ Virol (2019) 11: 32. https://doi.org/10.1007/s12560-019-09366-4
    Journal
    FOOD AND ENVIRONMENTAL VIROLOGY
    Rights
    © Springer Science+Business Media, LLC, part of Springer Nature 2019
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    The buffalo green monkey (BGM) cell line is required for the detection of enteric viruses in biosolids through a total culturable viral assay (TCVA) by the United States Environmental Protection Agency. In the present study, BGM and PLC/PRF/5 cell lines were evaluated for TCVA and for their use in determining the incidence of adenoviruses and enteroviruses in raw sludge and Class B biosolids. Six raw sludge and 17 Class B biosolid samples were collected from 13 wastewater treatment plants from seven U.S. states. Samples were processed via organic flocculation and concentrate volumes equivalent to 4 g total solids were assayed on BGM and PLC/PRF/5 cells. Cell monolayers were observed for cytopathic effect (CPE) after two 14-days passages. Cell lysates were tested for the presence of adenoviruses and enteroviruses by PCR or RT-PCR. The PLC/PRF/5 cells detected more culturable viruses than the BGM cells by CPE (73.9% vs. 56.5%, respectively). 52% of the samples were positive for CPE using both cell lines. No viruses were detected in either cell line by PCR in flasks in which CPE was not observed. No adenoviruses were detected in 13 CPE-positive samples from BGM lysates. In contrast, of the 17 samples exhibiting CPE on PLC/PRF/5 cells, 14 were positive for adenoviruses (82.4%). In conclusion, PLC/PRF/5 cells were superior for the detection of adenoviruses in both raw sludge and Class B biosolids. Thus, the use of BGM cells alone for TCVA may underestimate the viral concentration in sludge/biosolid samples.
    Note
    12 month embargo; published online: 23 January 2019
    ISSN
    1867-0342
    PubMed ID
    30673939
    DOI
    10.1007/s12560-019-09366-4
    Version
    Final accepted manuscript
    Additional Links
    https://link.springer.com/article/10.1007/s12560-019-09366-4
    ae974a485f413a2113503eed53cd6c53
    10.1007/s12560-019-09366-4
    Scopus Count
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