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dc.contributor.authorAbd-Elmaksoud, Sherif
dc.contributor.authorCastro-Del Campo, Nohelia
dc.contributor.authorGerba, Charles P
dc.contributor.authorPepper, Ian L
dc.contributor.authorBright, Kelly R
dc.date.accessioned2019-03-18T19:37:41Z
dc.date.available2019-03-18T19:37:41Z
dc.date.issued2019-03-01
dc.identifier.citationAbd-Elmaksoud, S., Castro-del Campo, N., Gerba, C.P. et al. Food Environ Virol (2019) 11: 32. https://doi.org/10.1007/s12560-019-09366-4en_US
dc.identifier.issn1867-0342
dc.identifier.pmid30673939
dc.identifier.doi10.1007/s12560-019-09366-4
dc.identifier.urihttp://hdl.handle.net/10150/631861
dc.description.abstractThe buffalo green monkey (BGM) cell line is required for the detection of enteric viruses in biosolids through a total culturable viral assay (TCVA) by the United States Environmental Protection Agency. In the present study, BGM and PLC/PRF/5 cell lines were evaluated for TCVA and for their use in determining the incidence of adenoviruses and enteroviruses in raw sludge and Class B biosolids. Six raw sludge and 17 Class B biosolid samples were collected from 13 wastewater treatment plants from seven U.S. states. Samples were processed via organic flocculation and concentrate volumes equivalent to 4 g total solids were assayed on BGM and PLC/PRF/5 cells. Cell monolayers were observed for cytopathic effect (CPE) after two 14-days passages. Cell lysates were tested for the presence of adenoviruses and enteroviruses by PCR or RT-PCR. The PLC/PRF/5 cells detected more culturable viruses than the BGM cells by CPE (73.9% vs. 56.5%, respectively). 52% of the samples were positive for CPE using both cell lines. No viruses were detected in either cell line by PCR in flasks in which CPE was not observed. No adenoviruses were detected in 13 CPE-positive samples from BGM lysates. In contrast, of the 17 samples exhibiting CPE on PLC/PRF/5 cells, 14 were positive for adenoviruses (82.4%). In conclusion, PLC/PRF/5 cells were superior for the detection of adenoviruses in both raw sludge and Class B biosolids. Thus, the use of BGM cells alone for TCVA may underestimate the viral concentration in sludge/biosolid samples.en_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.urlhttps://link.springer.com/article/10.1007/s12560-019-09366-4en_US
dc.rights© Springer Science+Business Media, LLC, part of Springer Nature 2019en_US
dc.subjectAdenovirusen_US
dc.subjectBGM cell lineen_US
dc.subjectBiosolidsen_US
dc.subjectEnteric virusesen_US
dc.subjectICC-PCRen_US
dc.subjectPLC cell lineen_US
dc.titleComparative Assessment of BGM and PLC/PRF/5 Cell Lines for Enteric Virus Detection in Biosolids.en_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Soil Water & Environm Scien_US
dc.identifier.journalFOOD AND ENVIRONMENTAL VIROLOGYen_US
dc.description.note12 month embargo; published online: 23 January 2019en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleFood and environmental virology


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