Seizure-Induced Arc mRNA Expression Thresholds in Rat Hippocampus and Perirhinal Cortex
AuthorChawla, Monica K.
Gray, Daniel T.
Huentelman, Matthew J.
Barnes, Carol A.
AffiliationUniv Arizona, Evelyn F McKnight Brain Inst
Univ Arizona, ARL Div Neural Syst Memory & Aging
Univ Arizona, Dept Psychol
Univ Arizona, Dept Neurol
Univ Arizona, Dept Neurosci
Keywordsin situ hybridization
calcium plateau potentials
MetadataShow full item record
PublisherFRONTIERS MEDIA SA
CitationChawla, M. K., Gray, D. T., Nguyen, C., Dhaliwal, H., Okuno, H., Huentelman, M. J., & Barnes, C. (2018). Seizure-induced Arc mRNA expression thresholds in rat hippocampus and perirhinal cortex. Frontiers in systems neuroscience, 12, 53.
RightsCopyright © 2018 Chawla, Gray, Nguyen, Dhaliwal, Zempare, Okuno, Huentelman and Barnes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractImmediate-early genes (IEGs) are rapidly and transiently induced following excitatory neuronal activity including maximal electroconvulsive shock treatment (ECT). The rapid RNA response can be blocked by the sodium channel antagonist tetrodotoxin ( I I X), without blocking seizures, indicating a role for electrical stimulation in electroconvulsive shock-induced mRNA responses. In behaving animals, Arc mRNA is selectively transcribed following patterned neuronal activity and rapidly trafficked to dendrites where it preferentially accumulates at active synapses for local translation. Here we examined whether there is a relationship between the current intensities that elicit seizures and the threshold for Arc mRNA transcription in the rat hippocampus and perirhinal cortex (PRC). Animals received ECT of varying current intensities (0, 20, 40 65, 77 and 85 mA) and were sacrificed 5 min later. While significantly more CA1, CA3 and perirhinal pyramidal cells expressed Arc at the lowest stimulus intensity compared to granule cells, there was an abrupt threshold transition that occurred in all four regions at 77 mA. This precise threshold for Arc expression in all temporal lobe neurons examined may involve regulation of the calcium-dependent mechanisms that are upstream to activity-dependent IEG transcription.
NoteOpen access journal.
VersionFinal published version
SponsorsMcKnight Brain Research Foundation; National Institutes of Health (NIH) [R01 AG048907, F31 AG055263]