• Login
    View Item 
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Pseudomonas aeruginosa increases MUC1 expression in macrophages through the TLR4-p38 pathway

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    MUC1_in_Mac-F-0712_kos.pdf
    Size:
    472.7Kb
    Format:
    PDF
    Description:
    Final Accepted Manuscript
    Download
    Author
    Kato, Kosuke
    Hanss, Alec D
    Zemskova, Marina A
    Morgan, Nicole E
    Kim, Marianne
    Knox, Kenneth S
    Lin, Yong
    Lillehoj, Erik P
    Kim, Kwang Chul
    Affiliation
    Univ Arizona, Coll Med, Dept Med
    Issue Date
    2017-10-14
    Keywords
    Inflammation
    MUC1
    Macrophage
    Membrane-tethered mucin
    Pseudomonas aeruginosa
    
    Metadata
    Show full item record
    Publisher
    ACADEMIC PRESS INC ELSEVIER SCIENCE
    Citation
    Kato, K., Hanss, A. D., Zemskova, M. A., Morgan, N. E., Kim, M., Knox, K. S., ... & Kim, K. C. (2017). Pseudomonas aeruginosa increases MUC1 expression in macrophages through the TLR4-p38 pathway. Biochemical and biophysical research communications, 492(2), 231-235.
    Journal
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Rights
    © 2017 Elsevier Inc. All rights reserved.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Alveolar macrophages (AMs) play a critical role in the clearance of Pseudomonas aeruginosa (Pa) from the airways. However, hyper-activation of macrophages can impair bacterial clearance and contribute to morbidity and mortality. MUC1 mucin is a membrane-tethered, high molecular mass glycoprotein expressed on the apical surface of mucosal epithelial cells and some hematopoietic cells, including macrophages, where it counter-regulates inflammation. We recently reported that Pa up-regulates the expression of MUC1 in primary human AMs and THP-1 macrophages, and that increased MUC1 expression in these cells prevents hyper-activation of macrophages that appears to be important for host defense against severe pathology of Pa lung infection. The aims of this study were to elucidate the mechanism by which Pa increases MUC1 expression in macrophages. The results showed that: (a) Pa stimulation of THP-1 macrophages increased MUC1 expression both at transcriptional and protein levels in a dose-dependent manner; (b) Both Pa- and LPS-induced MUC1 expression in THP-1 cells were significantly diminished by an inhibitory peptide of TLR4; and (c) LPS-stimulated MUC1 expression was diminished at both the mRNA and protein levels by an inhibitor of the p38 mitogen-activated protein kinase, but not by inhibitors of ERK1/2, JNK, or IKK. We conclude that Pa-stimulated MUC1 expression in THP-1 macrophages is regulated mainly through the TLR4-p38 signaling pathway. (C) 2017 Elsevier Inc. All rights reserved.
    Note
    12 month embargo; published online: 16 August 2017
    ISSN
    1090-2104
    PubMed ID
    28822766
    DOI
    10.1016/j.bbrc.2017.08.056
    Version
    Final accepted manuscript
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S0006291X1731608X?via%3Dihub
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bbrc.2017.08.056
    Scopus Count
    Collections
    UA Faculty Publications

    entitlement

    Related articles

    • Membrane-Tethered MUC1 Mucin Counter-Regulates the Phagocytic Activity of Macrophages.
    • Authors: Kato K, Uchino R, Lillehoj EP, Knox K, Lin Y, Kim KC
    • Issue date: 2016 Apr
    • Pseudomonas aeruginosa stimulates tyrosine phosphorylation of and TLR5 association with the MUC1 cytoplasmic tail through EGFR activation.
    • Authors: Kato K, Lillehoj EP, Kim KC
    • Issue date: 2016 Mar
    • Cutting edge: enhanced pulmonary clearance of Pseudomonas aeruginosa by Muc1 knockout mice.
    • Authors: Lu W, Hisatsune A, Koga T, Kato K, Kuwahara I, Lillehoj EP, Chen W, Cross AS, Gendler SJ, Gewirtz AT, Kim KC
    • Issue date: 2006 Apr 1
    • Membrane-tethered MUC1 mucin is phosphorylated by epidermal growth factor receptor in airway epithelial cells and associates with TLR5 to inhibit recruitment of MyD88.
    • Authors: Kato K, Lillehoj EP, Park YS, Umehara T, Hoffman NE, Madesh M, Kim KC
    • Issue date: 2012 Feb 15
    • Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against <i>Pseudomonas aeruginosa</i> lung infection.
    • Authors: Lillehoj EP, Guang W, Hyun SW, Liu A, Hegerle N, Simon R, Cross AS, Ishida H, Luzina IG, Atamas SP, Goldblum SE
    • Issue date: 2019 Jan 11
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.