Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae)
Affiliation
Univ Arizona, Sch Plant SciUniv Arizona, Dept Ecol & Evolutionary Biol
Issue Date
2018-05-16
Metadata
Show full item recordPublisher
MDPICitation
Huang Y-L, Zimmerman NB, Arnold AE. Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae). Journal of Fungi. 2018; 4(2):58.Journal
JOURNAL OF FUNGIRights
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Fungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, Populus trichocarpa, which has endophytes that represent three distantly-related genera (Cladosporium, Penicillium, and Trichoderma). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the interior of living leaves following in vivo inoculations, standard methods for isolating foliar endophytes confirmed their presence.Note
Open access journalISSN
2309-608XPubMed ID
29772709Version
Final published versionSponsors
Gordon and Betty Moore Foundation [GBMF 2550.03]; College of Agriculture and Life Science (CALS); School of Plant Sciences at the University of ArizonaAdditional Links
https://www.mdpi.com/2309-608X/4/2/58ae974a485f413a2113503eed53cd6c53
10.3390/jof4020058
Scopus Count
Collections
Except where otherwise noted, this item's license is described as © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Related articles
- Diversity, taxonomic composition, and functional aspects of fungal communities in living, senesced, and fallen leaves at five sites across North America.
- Authors: U'Ren JM, Arnold AE
- Issue date: 2016
- Common foliar fungi of Populus trichocarpa modify Melampsora rust disease severity.
- Authors: Busby PE, Peay KG, Newcombe G
- Issue date: 2016 Mar
- Canopy cover and leaf age affect colonization by tropical fungal endophytes: Ecological pattern and process in Theobroma cacao (Malvaceae).
- Authors: Arnold AE, Herre EA
- Issue date: 2003 May-Jun
- Simple methods to remove microbes from leaf surfaces.
- Authors: Saldierna Guzmán JP, Nguyen K, Hart SC
- Issue date: 2020 Aug
- Fungal endophytes limit pathogen damage in a tropical tree.
- Authors: Arnold AE, Mejía LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA
- Issue date: 2003 Dec 23