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dc.contributor.authorHuang, Yu-Ling
dc.contributor.authorZimmerman, Naupaka B
dc.contributor.authorArnold, A Elizabeth
dc.date.accessioned2019-05-22T20:00:54Z
dc.date.available2019-05-22T20:00:54Z
dc.date.issued2018-05-16
dc.identifier.citationHuang Y-L, Zimmerman NB, Arnold AE. Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae). Journal of Fungi. 2018; 4(2):58.en_US
dc.identifier.issn2309-608X
dc.identifier.pmid29772709
dc.identifier.doi10.3390/jof4020058
dc.identifier.urihttp://hdl.handle.net/10150/632372
dc.description.abstractFungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, Populus trichocarpa, which has endophytes that represent three distantly-related genera (Cladosporium, Penicillium, and Trichoderma). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the interior of living leaves following in vivo inoculations, standard methods for isolating foliar endophytes confirmed their presence.en_US
dc.description.sponsorshipGordon and Betty Moore Foundation [GBMF 2550.03]; College of Agriculture and Life Science (CALS); School of Plant Sciences at the University of Arizonaen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.urlhttps://www.mdpi.com/2309-608X/4/2/58en_US
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectCladosporiumen_US
dc.subjectPenicilliumen_US
dc.subjectPopulusen_US
dc.subjectTrichodermaen_US
dc.subjectcolonizationen_US
dc.subjectmicroscopyen_US
dc.subjectstomataen_US
dc.titleObservations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae)en_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Sch Plant Scien_US
dc.contributor.departmentUniv Arizona, Dept Ecol & Evolutionary Biolen_US
dc.identifier.journalJOURNAL OF FUNGIen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleJournal of fungi (Basel, Switzerland)
refterms.dateFOA2019-05-22T20:00:55Z


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