Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes
Koch, Lauren E
Isoe, Yurika E
Rascón, Alberto A
Brown, Heidi E
Massani, Brooke B
Miesfeld, Roger L
AffiliationUniv Arizona, Dept Chem & Biochem
Univ Arizona, Mel & Enid Zuckerman Coll Publ Hlth
MetadataShow full item record
PublisherPUBLIC LIBRARY SCIENCE
CitationIsoe J, Koch LE, Isoe YE, Rascón AA Jr, Brown HE, Massani BB, et al. (2019) Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes. PLoS Biol 17(1): e3000068. https://doi.org/10.1371/journal.pbio.3000068
Rights© 2019 Isoe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
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AbstractMosquito-borne diseases are responsible for several million human deaths annually around the world. One approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. To this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. We performed bioinformatic analyses to identify putative protein-coding sequences that are specific to mosquito genomes. Systematic RNA interference (RNAi) screening of 40 mosquito- specific genes was performed by injecting double-stranded RNA (dsRNA) into female Aedes aegypti mosquitoes. This experimental approach led to the identification of eggshell organizing factor 1 (EOF1, AAEL012336), which plays an essential role in the formation and melanization of the eggshell. Eggs deposited by EOF1-deficient mosquitoes have nonmelanized fragile eggshells, and all embryos are nonviable. Scanning electron microscopy (SEM) analysis identified that exochorionic eggshell structures are strongly affected in EOF1-deficient mosquitoes. EOF1 is a potential novel target, to our knowledge, for exploring the identification and development of mosquito-selective and biosafe small-molecule inhibitors.
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VersionFinal published version
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